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| 应用RAMP分子标记探讨仲彬草属的种间关系 | |
| 引用本文： | 张利,永红,魏育明,郑有良,刘世贵. 应用RAMP分子标记探讨仲彬草属的种间关系[J]. 高技术通讯, 2003, 13(4): 28-33 |
| 作者姓名： | 张利 永红 魏育明 郑有良 刘世贵 |
| 作者单位： | 1. 四川大学草原生物防治国家实验室,成都,610064;四川农业大学小麦研究所,都江堰,611830 2. 四川农业大学小麦研究所,都江堰,611830 3. 四川大学草原生物防治国家实验室,成都,610064 |
| 基金项目： | 国家自然科学基金(30270099)，四川省教育厅和科技厅重点基金资助项目。 |
| 摘 要： | 对仲彬草属Kengyilia14个种和1个变种进行了RAMP分析。结果表明物种间遗传差异明显。40个引物组合产生的254条DNA扩增片段中，216条(85．0％)具有多态性，每个引物组合可扩增出l一9条多态性带，平均5．4条。遗传相似系数变化范围为0．327—0．886，平均值为0．549。同时，形态相似、地理分布一致的物种有一定的亲缘关系，聚类在一起。这与RAPD、形态学和细胞学等分析结果基本一致。因此，RAMP评分子标记是评价仲彬草属种间关系十分有效的方法。 |
| 关 键 词： | RAMP分子标记 仲彬草属 种间关系 形态学 细胞学 遗传变异 亲缘关系 |
Relationships among Kengyilia Species Based on Random Amplified Microsatellite Polymorphism (RAMP) |
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| Zhang Li,Zhou Yonghong,Wei Yuming,Zheng Youliang,Liu Shigui. Relationships among Kengyilia Species Based on Random Amplified Microsatellite Polymorphism (RAMP)[J]. High Technology Letters, 2003, 13(4): 28-33 | |
| Authors: | Zhang Li Zhou Yonghong Wei Yuming Zheng Youliang Liu Shigui |
| Abstract: | Relationships among 14 Kengyilia species and 1 variety were analyzed using random amplified microsatellite polymorphism (RAMP) markers. The results showed distinct genetic differences were present among the species. 40 primer combinations produced 254 bands, among which 216 out of 254 bands (85.0%) were polymorphic. 1-9 polymorphic bands could be amplified from each primer combination, with an average of 5.4 bands. The genetic similarity coefficients ranged from 0.327 to 0.886, with the mean of 0.549. Meantime, the species with similar morphological characters and the species from the same areas or neighboring geographical regions were clustered together, which suggested that had close relationships. RAMP results are basically comparable with those obtained from studies on RAPD, morphology and cytology. It is a useful method for analysis of the relationship in Kengyilia. |
| Keywords: | Kengyilia RAMP analysis Relationship |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |