Adaptively controlled supercontinuum pulse from a microstructure fiber for two-photon excited fluorescence microscopy |
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Authors: | Tada Junji Kono Taiki Suda Akira Mizuno Hideaki Miyawaki Atsushi Midorikawa Katsumi Kannari Fumihiko |
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Affiliation: | Department of Electronics and Electrical Engineering, Keio University, 3-14-1, Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan. |
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Abstract: | Selective fluorescence excitation of specific molecular species is demonstrated by using coherent control of two-photon excitation with supercontinuum pulses generated with a microstructure fiber. Pulse shaping prior to pulse propagation through the fiber is controlled by a self-learning optimization loop so that the highest fluorescence signal contrast between two fluorescent proteins is obtainable. The self-learning optimization loop successfully controls both the optical nonlinarity of the microstructure fiber and the two-photon excitation of the fluorescent proteins. |
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