Effects of biodegradable Mg-6Zn alloy extracts on apoptosis of intestinal epithelial cells |
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Authors: | Zhanhui Wang Jun Yan Jianan LiQi Zheng Zhigang WangXiaonong Zhang Shaoxiang Zhang |
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Affiliation: | a Shanghai Jiao Tong University School of Medicine, 227 South Chongqing Road, Shanghai 200025, PR China b Department of General Surgery, Sixth People's Hospital, Shanghai Jiao Tong University, 600 Yishan Road, Shanghai 200233, PR China c State Key Laboratory of Metal Matrix Composites, Institute of Biomedical Materials, School of Materials Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, PR China d Suzhou Origin Medical Technology Co. Ltd., Jiangsu 215513, PR China |
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Abstract: | In this study, intestinal epithelial cells (IEC)-6 were cultured in different concentration extracts of Mg-6Zn alloys for different time periods. To achieve a total of three concentrations (100%, 60% and 20% concentration), the extracts were serially diluted with Dulbecco's modified Eagle medium High Glucose to observe a dose-response relationship. We studied the indirect effects of Mg-6Zn alloys on IEC-6 cells apoptosis. The apoptosis of IEC-6 cells was measured using flow cytometry. And the apoptosis of IEC-6 cells was evaluated by investigating the expression of caspase-1and Bcl-2 using real-time polymerase chain reaction (PCR) and Western blotting tests. It was found that the levels of apoptosis in IEC-6 cells cultured in 100% Mg-6Zn alloy extracts were significantly higher than those in 60% and 20% extracts; the 100% extract can down-regulate expression of Bcl-2 after culture. The in vitro results indicated that the conspicuous alkaline environment and excessive Mg concentration, even Zn concentration caused by rapid corrosion of Mg-6Zn alloys promote IEC-6 cells apoptosis, although further experiments will be necessary to formally prove our conclusions. Therefore, the adjustment of the degradation rate is needed for using Mg-Zn alloy as a surgical suture material. |
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Keywords: | Magnesium Apoptosis Caspase-1 Bcl-2 In vitro test |
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