High-throughput microfluidic systems for disease detection |
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Authors: | Pin-Chuan Chen Kuan-Chin Kuo |
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Affiliation: | 1. Department of Mechanical Engineering , National Taiwan University of Science and Technology , Taipei , Taiwan, ROC pcchen@mail.ntust.edu.tw;3. Department of Mechanical Engineering , National Taiwan University of Science and Technology , Taipei , Taiwan, ROC |
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Abstract: | Accuracy and rapid response are critical to the detection of an acute infectious disease, not only because the detection results can affect the medical treatment, but also can prevent disease outbreaks. Since the current culture-based technology is time consuming and experience dependent, academia and industrial researchers are using microfluidics and nucleic acids as the fundamental ideas to build pioneering tools against infectious disease. While many point-of-care microfluidic systems have been realized to execute nucleic acid applications, high-throughput microfluidic systems are under development for various nucleic acid applications because of high efficiency and demand from the market. Building a high-throughput system is an interdisciplinary challenge because of the design concerns from science and the manufacturing concerns from engineering, but its realization will be a milestone. This article is aimed to review three essential steps of the nucleic acid-based detection realized in high-throughput formats, including polymerase chain reaction, capillary electrophoresis, and nucleic acid purification. |
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Keywords: | high-throughput microfluidic system micro total analysis systems (μTAS) point-of-care microfluidics microfluidics |
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