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| 酶-二维MXene复合材料的制备及其电化学检测H2O2的应用 | |
| 引用本文： | 马保凯,李勉,张绫芷,翁新楚,沈彩,黄庆.酶-二维MXene复合材料的制备及其电化学检测H2O2的应用[J].无机材料学报,2020,35(1):131-138. |
| 作者姓名： | 马保凯 李勉 张绫芷 翁新楚 沈彩 黄庆 |
| 作者单位： | 上海大学 生命科学学院, 上海 200444 宁波大学 食品与药学学院, 宁波 315211 中国科学院 宁波材料技术与工程研究所, 宁波 315201 |
| 摘 要： | 本研究合成了具有垂直栅栏结构的二维MXene材料, 与辣根过氧化物酶进行固定, 构筑了过氧化氢电化学酶传感器。合成的MXene纳米栅栏具有大的比表面积, 优良的电子传导特性和在水溶液中的良好分散特性; 固定化在酶电极上的辣根过氧化物酶分子表现出了优良的过氧化氢催化效果。结果表明HRP@MXene/chitosan/GCE酶电化学传感器在过氧化氢浓度为5~1650 μmol/L范围内表现出很好的线性关系, 最低检测限为0.74 μmol/L, 且具有很好的操作稳定性, 该生物传感器被成功地应用于固态与液态食品中过氧化氢残留检测。 |
| 关 键 词： | 辣根过氧化物酶 MXene纳米片 生物传感器 过氧化氢 |
| 收稿时间： | 2019-03-28 |
Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2 |
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| MA Bao-Kai,LI Mian,CHEONG Ling-Zhi,WENG Xin-Chu,SHEN Cai,HUANG Qing.Enzyme-MXene Nanosheets: Fabrication and Application in Electrochemical Detection of H2O2[J].Journal of Inorganic Materials,2020,35(1):131-138. | |
| Authors: | MA Bao-Kai LI Mian CHEONG Ling-Zhi WENG Xin-Chu SHEN Cai HUANG Qing |
| Affiliation: | School of Life and Sciences, Shanghai University, Shanghai 200444, China Department of Food Science and Engineering, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315211, China Institute of Materials Technology & Engineering, Chinese Academy of Sciences, Ningbo 315201, China |
| Abstract: | Two-dimensional MXene nanosheets with vertical junction structure was employed for easy immobilization of horse radish peroxidase enzymes to fabricate the electrochemical hydrogen peroxide (H2O2) biosensor. The synthesized MXene nanosheets exhibited large specific area, excellent electronic conductivity and good dispersion in aqueous phase. Horse Radish Peroxidase (HRP) enzymes molecules immobilized on MXene/chitosan/GCE electrode demonstrated good electrocatalytic activity toward reduction of H2O2. The fabricated HRP@MXene/chitosan/GCE biosensor exhibited a wide linear range from 5 to 1650 μmol?L -1, a limit of detection of 0.74 μmol?L -1 and good operation stability. The fabricated biosensor was successfully employed for detection of trace level of H2O2 in both solid and liquid food. |
| Keywords: | horse radish peroxidase MXene nanosheets biosensor hydrogen peroxide |
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