| 液相色谱串联质谱法测定烟草中的游离氨基酸 |
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| 引用本文： | 马戎,鲍峰伟,冯文宁,解民,张艳芳,陈伟华.液相色谱串联质谱法测定烟草中的游离氨基酸[J].中国测试,2013(2):34-37. |
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| 作者姓名： | 马戎 鲍峰伟 冯文宁 解民 张艳芳 陈伟华 |
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| 作者单位： | 河北中烟工业有限责任公司技术中心 |
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| 摘 要： | 建立液相色谱串联质谱法测定烟草中20种游离氨基酸的方法。烟草样品经0.1%的盐酸溶液超声萃取并离心后,直接进样测定。色谱柱采用XTerra MS C18(50mm×2.1mm×2.5μm),0.1%甲酸溶液和乙腈为流动相。结果表明:20种氨基酸的检出限为0.001～0.011μg/mL,标准曲线的拟合度均大于0.999,回收率在86.4%～105.9%之间。该方法操作简单,灵敏度高,适用于烟草中游离氨基酸的检测。
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| 关 键 词： | 液质联用 游离氨基酸 烟草 超声 |
Determination of free amino acids in tobacco by liquid chromatography tandem mass spectrometry |
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| Authors: | MA Rong BAO Feng-wei FENG Wen-ning XIE Min ZHANG Yan-fang CHEN Wei-hua |
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| Affiliation: | (China Tobacco Hebei Industrial Corporation Technology Center,Shijiazhuang 050035,China) |
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| Abstract: | A method for determining of 20 free amino acids in tobacco was developed with liquid chromatography tandem mass spectrometry.Tobacco samples were extracted with 0.1% hydrochloric acid solution by ultrasonic.After centrifugal separation treatment,they were injected into the instrument and determined divectly.The LC separation was carried on a reversed-phase XTerra MS C18(50 mm×2.1 mm×2.5 μm),0.1% formic acid solution and acetonitrile were taken as mobile phase.The results showed that the limits of detection of 20 amino acids were in the range of 0.001-0.011 μg/mL,the linear correlation coefficients were above 0.999 in all cases,and the the recovery were in the range of 86.4%-105.9%.This method is simple operation,high sensitivity,and suitable for determining the free amino acid in tobacco. |
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| Keywords: | LC/MS free amino acid tobacco ultrasonic |
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