Ti-HA-BaTiO_3生物复合材料的制备与性能

为进一步提高骨修复材料的骨诱导性能,引入了BaTiO_3压电涂层。首先,通过等离子喷涂法在Ti基体上制备羟基磷灰石(HA)、Ti-HA和Ti-HA-BaTiO_3这3种生物涂层;然后,采用正交试验设计优化了喷涂工艺参数,用SEM/EDS对涂层的形貌和成分进行了表征,并通过划痕试验测试了涂层与基体的结合强度;最后,进行了细胞培养试验,用CCK-8法对细胞毒性进行了评价,并在SEM下观察了细胞的粘附形态。结果显示:细胞带有伪足,呈梭形和不规则多边形,且粘附在Ti-HA-BaTiO_3复合涂层表面;细胞伸展形态良好,涂层的细胞毒性等级不高于1级。可见,Ti-HA-BaTiO_3复合材料可以作为临床植入材料应用。

Preparation and properties of Ti-HA-BaTiO3 bio-composites

In order to further improve the bone inductive properties of bone repair materials, BaTiO₃piezoelectric coating was introduced.First, three kinds of biological coatings of hydroxyapatite (HA), Ti-HA and Ti-HA-BaTiO₃ were prepared on Ti substrates by plasma spraying method. Then, the spray process parameters were optimized by orthogonal test design, the morphology and composition of coatings were characterized by SEM/EDS, and the bonding strength between coating and substrate was tested by scratch test. Finally, the cell culture test was conducted, the cytotoxicity was evaluated by CCK-8 method, and the adhesion morphology of cells was observed under SEM. The results show that cells have pseudopodia, present fusiform and irregular polygons, and adhere to the surface of Ti-HA-BaTiO₃ composite coating; cells spread in good shape, the cytotoxicity level of coating is no higher than Level 1.It follows that Ti-HA-BaTiO₃bio-composites can be used as clinical implant materials.