异丙酚预处理对缺氧/复氧后培养海马神经元金属硫蛋白-3蛋白表达的影响

目的:本研究观察异丙酚对培养海马神经元缺氧复氧后损伤反应的影响及金属硫蛋白-3(metallothionein-3,MT-3)在其中的作用。方法: 培养7~10 d 海马神经元,以不同浓度的异丙酚(50,150,250 μmol/L)预处理后 24 h 后,制备缺氧 4 h 后复氧 24 h 模型(H/R),Western blot法检测MT-3蛋白表达水平;以MT-3特异性siRNA沉默MT-3表达后,采用MTT法观察异丙酚对H/R海马神经元存活率的影响。结果: 在H/R条件下,异丙酚可浓度依赖性促进MT-3的蛋白表达。以MT-3 siRNA抑制MT-3蛋白表达后,异丙酚提高神经元存活率的作用消失。结论:异丙酚具有神经元保护效应,这一作用可能与其上调MT-3的蛋白表达有关。

The impact of propofol pretreatment on hypoxia/reoxygenation in cultured hippocampal neurons metallothionein-3 protein expression

AIM: To observe the effects of propofol on caltured hippocampal neurons hypoxia and reoxygenation damage response and metallothionein protein-3(metllothionein-3,MT-3) in which the role.METHODS: Hippocampal neuron cells were cultured for 7-10 days for use. Hypoxic conditions were attained 4 h and reoxygenation was achieved with normoxia conditions for another 24 h. The expression level of metallothionein-3 (MT-3) was detected by Western blot; By using MT-3 specific siRNA, MT-3 was silenced and the effect of propofol on cell survival rate was evaluated by MTT method .RESULTS:In cultured hippocampal neuron cells, pretreatment with different concentrations of propofol (50, 150, 250 mol/L) for 24 h enhanced MT-3 expression level after hypoxia/reoxygenation. When MT-3 expression was silenced by specific siRNA, propofol failed to improve hippocampal neuron cell survival rate.CONCLUSION: MT-3 exerts hippocampal neuron protective effect, which may possibly be mediated by the upreguation of MT-3 expression.