circZNF124通过靶向miR-4262调控结直肠癌SW620细胞增殖、迁移及侵袭的机制研究

目的：探讨circZNF124对结直肠癌SW620细胞增殖、迁移及侵袭的影响及其机制。方法：体外培养人结直肠癌细胞SW620，随机分组：si-NC组、si-circZNF124组、miR-NC组、miR-4262组、si-circZNF124+anti-miR-NC组、si-circZNF124+anti-miR-4262组；CCK-8法、平板克隆形成实验、划痕实验与Transwell实验分别检测SW620细胞增殖、克隆形成、迁移及侵袭；双荧光素酶报告实验分析circZNF124与miR-4262的靶向结合；Western blot检测E-cadherin、N-cadherin蛋白表达量。结果：与si-NC组比较，si-circZNF124组细胞活力、划痕愈合率和N-cadherin蛋白水平降低（P<0.05），细胞克隆形成数和侵袭细胞数减少（P<0.05），E-cadherin蛋白水平升高（P<0.05）；circZNF124可负向调控miR-4262的表达；与miR-NC组比较，miR-4262组细胞活力、划痕愈合率和N-cadherin蛋白水平降低（P<0.05），细胞克隆形成数和侵袭细胞数减少（P<0.05），E-cadherin蛋白水平升高（P<0.05）；抑制miR-4262表达逆转了干扰circZNF124表达对SW620细胞增殖、迁移侵袭的作用。 结论：干扰circZNF124表达通过靶向miR-4262，减弱结直肠癌细胞增殖、迁移及侵袭能力。

circZNF124 regulates the proliferation,migration and invasion of colorectal cancer SW620 cells by targeting miR-4262

AIM: To explore the effect and mechanism of circZNF124 on the proliferation, migration and invasion of colorectal cancer SW620 cells. METHODS: The expression levels of circZNF124 and miR-4262 in colorectal cancer tissues were measured by qRT-PCR method. Human colorectal cancer cells SW620 were cultured in vitro, and were randomly grouped into si-NC group, si-circZNF124 group, miR-NC group, miR-4262 group, si-circZNF124+anti-miR-NC group, si-circZNF124+anti-miR-4262 groups. CCK-8 method, plate clone formation test, scratch test and Transwell test respectively were used to detect cell proliferation, clone formation, migration and invasion of SW620 cells. The dual luciferase reporter experiment analyzed the targeted binding of circZNF124 to miR-4262. Western blot was used to detect the expression of E-cadherin and N-cadherin protein. RESULTS: The expression of circZNF124 in colorectal cancer tissue was increased by about 3.75 times compared with that in the adjacent tissue (P<0.05), and the expression of miR-4262 was decreased by about 0.73 times compared with the adjacent tissue (P<0.05). Compared with the si-NC group, the cell viability, scratch healing rate and the protein level of N-cadherin in the si-circZNF124 group were decreased (P<0.05), the number of cell clone formation and the number of invasive cells were decreased (P<0.05), while the protein level of E-cadherin was increased (P<0.05). circZNF124 could negatively regulate the expression of miR-4262. Compared with the miR-NC group, the cell viability, scratch healing rate and the protein level of N-cadherin in the miR-4262 group were reduced (P<0.05), the number of cell clones and the number of invasive cells were reduced (P<0.05), while the protein level of E-cadherin was increased (P<0.05). Inhibition of miR-4262 expression reversed the effect of interfering circZNF124 expression on the proliferation, migration and invasion of SW620 cells.CONCLUSION: Interference with the expression of circZNF124 can attenuate the proliferation, migration and invasion of colorectal cancer cells by targeting miR-4262.