参麦注射液对肺缺血-再灌注损伤时Fas/FasL 表达的影响

目的 探讨参麦注射液对肺缺血-再灌注损伤(PIRI) 时Fas/FasL 配体(Fas/FasL) 表达的影响。方法 采用在体兔单肺原位缺血-再灌注模型。实验兔30 只, 随机分为假手术对照组(sham,n=10) 、肺缺血-再灌注组(I-R, n=10) 和肺缺血-再灌注加参麦注射液组(SM, n=10)。分别于再灌注3 h 取左肺组织, 观察Fas/FasL mRNA 定位表达、凋亡指数(AI) 、肺组织湿干重比(W D) 、肺损伤组织学定量评价指标(IQA) 及光镜、电镜下的组织形态学改变。结果 SM 组Fas/FasL mRNA在肺小动脉内(外) 膜、肺小静脉内膜、肺泡上皮及肺支气管上皮呈弱阳性表达, 明显低于I-R 组(P<0.05);AI 、W D 和IQA 值显著低于I-R 组(P <0.01 和P <0.05);肺组织形态学异常改变程度减轻。结论 参麦注射液可下调肺组织Fas/FasLmRNA 的表达而减轻细胞凋亡, 对PIRI 发挥积极的防治作用。

Effects of Shenmai injection on the expression of Fas/FasL mRNA in pulmonary injury induced by ischemia-reperfusion in rabbits

AIM: To investigate the effects of Shenmai injection (SM) on the expression of Fas/FasL mRNA during pulmonary ischemia and reperfusion injury (PIRI) in rabbits.METHODS: Lung ischemia and reperfusion animal orthotopic model was established in vivo.The rabbits were randomly divided into three groups:sham operated group (sham, n=10), ischemia-reperfusion group (I-R, n=10) and I-R +Shenmai injection group (SM, n=10).The changes in several parameters, which included Fas/FasL mRNA locating expression, apoptotic index (AI), wet to dry ratio of lung tissueweight(W D) and index of quantitative assessment of histologic lung injury(IQA), and the histomorphology under light and electron microscope, were evaluated at 3 h after reperfusion in lung tissue.RESULTS: Compared with I-R group, the Fas/FasL mRNA slightly expressed in intima and extima of small pulmonary artery, pulmonary vein, and alveolar epithelium, bronchiole epithelia in SM group.The values of AI, W D and IQA were significantly lower than those in I-R group at 3 h after reperfusion in lung tissue (P <0.01 and P <0.05), and the changes of lung tissue in histomorphology were less markedly in SM group.CONCLUSION: Shenmai injection has notable protective effects on PIRI in rabbits, which may related with inhibition on Fas/FasL mRNA expression in lung tissue and decreasing apoptosis.