| 当归挥发油对人肺腺癌GLC-82细胞增殖及凋亡的影响 |
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| 引用本文： | 张艳霞,蔺兴遥,安方玉,张正顺,陈绍仪,周佳玮,张延英.当归挥发油对人肺腺癌GLC-82细胞增殖及凋亡的影响[J].金属学报,2018,23(1):29-33. |
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| 作者姓名： | 张艳霞 蔺兴遥 安方玉 张正顺 陈绍仪 周佳玮 张延英 |
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| 作者单位： | 1.甘肃中医药大学/甘肃省中药质量与标准研究重点实验室培育基地，兰州 730000，甘肃； ;2.甘肃省中医院白银分院，白银 730900，甘肃；;3.白银市第二人民医院，白银 730900，甘肃 |
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| 基金项目： | 白银市科技计划项目（2013-2-76Y）；甘肃中医学院中青年科研基金项目（2305016601） |
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| 摘 要： | 目的: 探讨当归挥发油对人肺腺癌GLC-82细胞增殖及凋亡的作用。方法: MTT比色法检测当归挥发油对GLC-82细胞的细胞毒作用；流式细胞术检测GLC-82细胞凋亡；吖啶橙/溴化乙锭（AO/BE）双荧光染色法观察GLC 82细胞凋亡。结果: 当归挥发油在浓度6.25～200 μg/mL范围内对GLC-82细胞的增殖具有浓度依赖性抑制作用，对GLC-82细胞的IC50为113.03 μg/mL。流式细胞术检测结果表明，当归挥发油可诱导GLC-82细胞凋亡、坏死，且细胞凋亡、坏死率随药物浓度增高而升高。AO/BE双荧光染色法结果显示，用药组随着药物浓度的升高，细胞结构固缩加重，凋亡、坏死细胞增多。结论: 当归挥发油具有诱导GLC-82细胞凋亡的作用。
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| 关 键 词： | 当归挥发油 人肺腺癌GLC-82细胞 细胞凋亡 |
| 收稿时间： | 2016-10-20 |
| 修稿时间： | 2016-11-30 |
Effects of volatile oil of the angelica on the proliferation and apoptosis of human lung adenocarcinoma cell GLC-82 |
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| ZHANG Yanxia,LIN Xingyao,AN Fangyu,ZHANG Zhengshun,CHEN Shaoyi,ZHOU Jiawei,ZHANG Yanying.Effects of volatile oil of the angelica on the proliferation and apoptosis of human lung adenocarcinoma cell GLC-82[J].Acta Metallurgica Sinica,2018,23(1):29-33. |
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| Authors: | ZHANG Yanxia LIN Xingyao AN Fangyu ZHANG Zhengshun CHEN Shaoyi ZHOU Jiawei ZHANG Yanying |
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| Affiliation: | 1.Gansu University of Traditional Chinese Medicine/ Key Laboratory of TCM Quality and Standard of Gansu Province, Lanzhou 730000, Gansu, China;2. Baiyin Branch of Gansu Province Hospital of Traditional Chinese Medicine, Baiyin 730900, Gansu, China;3.The Second People's Hospital of Baiyin, Baiyin 730900, Gansu, China |
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| Abstract: | AIM: To investigate the cytotoxic effect and apoptosis of volatile oil of the angelica on human lung adenocarcinoma cell GLC-82. METHODS: MTT colorimetric assay was used to investigate the cytotoxic effect of human lung adenocarcinoma cell GLC-82. The cell apoptosis was analyzed by flow cytometry. AO/BE fluorescent staining was applied to observe the cell apoptosis. RESULTS: The GLC-82 cell was inhibited by volatile oil of the angelica in a concentration-dependent manner; the IC50 was 113.03 μg/mL. The result of flow cytometry indicated volatile oil of the angelica could induce apoptosis and necrosis of GLC-82 cell, and the rates of apoptosis and necrosis were increased by the increase of drug concentration. The results of AO/BE fluorescent staining method indicated the rate of apoptosis and necrosis increased in drug group along with the increase of drug concentration. CONCLUSION: Volatile oil can induce the apoptosis of GLC-82 cell. |
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| Keywords: | volatile oil of the angelica human lung adenocarcinoma cell GLC-82 apoptosis |
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