调节性B细胞在自身免疫性溶血性贫血患者外周血中的表达及临床意义

目的: 探讨自身免疫性溶血性贫血（AIHA）患者外周血中调节性B细胞（Bregs）的表达及其在该病发病中的意义。方法:选择16例自身免疫性溶血性贫血患者和14例健康志愿者为研究对象，用流式细胞术分析外周血CD19+IL-10+调节性B细胞及CD19+CD24hiCD27+调节性B细胞的表达；ELISA方法检测培养上清液中IL-10的水平。结果:自身免疫性溶血性贫血患者外周血中CD19+IL-10+调节性B细胞、CD19+CD24hiCD27+调节性B细胞的表达分别为（1.27±0.39）％、（9.85±2.18）％，健康志愿者组分别为（2.92±0.71）％、（26.47±4.31）％，两组比较差异有统计学意义（P<0.05）；自身免疫性溶血性贫血患者细胞培养上清中IL-10的水平低于健康志愿者组（P<0.05）。结论:自身免疫性溶血性贫血患者外周血中CD19+IL-10+调节性B细胞及CD19+CD24hiCD27+调节性B细胞比例降低，提示调节性B细胞可能参与自身免疫性溶血性贫血的发病过程。

Number of regulatory B cells in peripheral blood of patients with autoimmune hemolytic anemia and its clinical signifcance

AIM: To explore the number of regulatory B cells (Bregs) in peripheral blood of patients with autoimmune hemolytic anemia (AIHA) and its significance in the incidence of AIHA. METHODS: A total of 16 AIHA patients and 14 healthy controls were enrolled. The expressions of CD19+IL-10+ Bregs and CD19+CD24hiCD27+ Bregs in peripheral blood mononuclear cells (PBMCs) were evaluated by flow cytometry. And levels of IL-10 were measured in the culture supermatants by ELISA. RESULTS: The expression of CD19+IL-10+ Bregs in PBMCs of AIHA patients was (1.27±0.39)%, which was lower than that in healthy controls (2.92±0.71)% (P<0.05). The number of CD19+CD24hiCD27+ Bregs in AIHA patients was (9.85±2.18)%, which was lower than that in healthy controls (26.47±4.31)% (P<0.05). The level of IL-10 significantly decreased in culture supermatants of autoimmune hemolytic anemia patient as compared with healthy controls (P<0.05). CONCLUSION: The expression of Bregs significantly decreases in PBMCs of AIHA patients. Bregs may play some role in the pathogenesis of AIHA.