睑板腺癌中MMP-2的表达及其临床意义

目的: 探讨基质金属蛋白酶-2(matrix metallop roteinase-2,MMP-2)在睑板腺囊肿和睑板腺癌组织中的表达差异及其与睑板腺癌临床病理特征的关系和临床意义。方法: 采用免疫组化SP法检测睑板腺囊肿和睑板腺癌组织中MMP-2、血管内皮生长因子(VEGF)的表达情况,以CD34标记检测肿瘤内的微血管密度(microvessel density, MVD),分析MMP-2与临床病理因素、VEGF、MVD的关系。结果: MMP-2在睑板腺癌组织中阳性表达率高于睑板腺囊肿(56% vs 20%),差异具有统计学意义(P﹤0.05),而VEGF表达在睑板腺癌和睑板腺囊肿中差别无统计学意义;MMP-2蛋白表达与肿瘤复发和局部浸润有关 (P﹤0.05),与患者年龄、组织学分级、病理类型和病程无关 (P﹥0.05)。MMP-2阳性的肿瘤组织内MVD明显高于MMP-2阴性肿瘤(P﹤0.05),VEGF的表达与MMP-2无相关性(r=-0.049,P=0.748)。结论: MMP-2在睑板腺癌中高表达,可能通过非VEFG依赖途径促进肿瘤的血管生成,进而参与肿瘤浸润和复发。

Expression and clinical significant of matrix metalloproteinase-2 in sebaceous carcinoma

AIM: To investigate the differential expression of MMP-2 between chalazion and sebaceous carcinoma tissues and explore the association of expression of MMP-2 with clinical pathological features and its clinical significance.METHODS: The expression of MMP-2 and VEGF in chalazion (20 cases) and sebaceous carcinoma tissues (50 cases) were detected using immunohistochemical method of SP.The microvessel density (MVD) was calculated by CD34 immunostaining in the tumor.The relationship between MMP-2 and clinicopathological characteristics, VEGF and MVD were analyzed.RESULTS: The positive expression rates of MMP-2(56%) in sebaceous carcinoma tissues were higher when compared with chalazion tissues(20%), the difference was statistically significant (P<0.05),but there was no difference in the expression of VEGF between sebaceous carcinoma and chalazion tissues.The expression of MMP-2 was positively correlated with recurrence and local infiltration in sebaceous carcinoma (P<0.05), while there was no significant association between expression of MMP-2 and clinical characteristics including age, histological grade, pathological type and duration of unrelated (P>0.05).Though MVD in MMP-2-positive tumors was higher than that in MMP-2-negative tumors (P<0.05), the expression of VEGF was not correlated with MMP-2 (r=-0.049,P=0.748).CONCLUSION: MMP-2 was over-expressed in sebaceous carcinoma tissues and may be involved in the progress of invasion and recurrence in sebaceous carcinoma via a VEFG-independent way to promote tumor angiogenesis.