Comparison of steam-alkali-chemical and microwave-alkali pretreatment for enhancing the enzymatic saccharification of oil palm trunk |
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| Affiliation: | 1. Faculty of Science and Biotechnology, Universiti Selangor, Bestari Jaya 45600, Malaysia;2. Department of Bioprocess Engineering, Faculty of Chemical Engineering, c/o Institute of Bioproduct Development, Universiti Teknologi Malaysia, UTM, Skudai 81310, Malaysia;1. Department of Electrical Engineering, M.I.T.S., Gwalior, India;2. Department of Electrical Engineering, IIT Delhi, India;1. Institut national de la recherche scientifique, Centre Eau Terre Environnement, 490 rue de la Couronne, Québec, QC, G1K 9A9, Canada;2. Département de génie mécanique, École de Technologie Supérieure, 1100 rue Notre-Dame Ouest, Montréal, QC, H3C 1K3, Canada;1. Department of Materials Science and Engineering, China Jiliang University, Hangzhou 310018, PR China;2. Zhejiang Tianneng Energy Technology Co., Ltd., Changxing County, Zhejiang Province 313100, PR China;1. Department of Electrical Engineering, College of Engineering, King Saud University, Riyadh, 11421, Saudi Arabia;2. Electrical & Computer Engineering, University of Waterloo, Waterloo, Ontario, N2L 3G1, Canada |
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| Abstract: | This paper demonstrates two different pretreatment protocols for oil palm trunks (OPT); steam-alkali-chemical (SAC) and microwave-alkali (Mw-A) method. The composition, morphology, structure and crystallinity of OPT before and after pretreatment were analyzed. The effectiveness of the pretreated methods was investigated by performing enzymatic saccharification on the OPT. The physiochemical factors namely: enzyme ratio (cellulase to β-glucosidase), pH, temperature and substrate loading (w/v) on enzymatic saccharification were also investigated. The pre-determined optimal conditions were then used for further enzymatic hydrolysis of raw and pretreated OPT substrates. The results revealed a huge degree of reduction in lignin, up to 89% for SAC treated OPT and at least 15% for Mw-A treated OPT sample as compared to untreated ones. High glucose accumulation (79.4%) was obtained after 72 h saccharification for both pretreated OPT samples. |
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| Keywords: | Cellulase Composition determination Crystallinity index Lignocellulose Morphology analyses Structure characterization |
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