Insights into biological delignification of rice straw by Trametes hirsuta and Myrothecium roridum and comparison of saccharification yields with dilute acid pretreatment |
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| Affiliation: | 1. Indian Agricultural Research Institute, Division of Microbiology, New Delhi 110012, India;2. Department of Food Science, University of Arkansas, Fayetteville, AR, USA;3. Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, AR, USA;1. Department of Agricultural and Biological Engineering, University of Illinois at Urbana-Champaign, 1304 W. Pennsylvania Avenue, Urbana, IL 61801, USA;2. Department of Food Science, University of Arkansas, 1180 W Cassatt St., Fayetteville, AR 72704, USA;3. Department of Biological and Agricultural Engineering, University of Arkansas, 203 Engineering Hall, Fayetteville, AR 72701, USA;1. College of Engineering, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning, 110866, China;2. Department of Chemical and Biological Engineering, University of Saskatchewan, 57 Campus Drive, Saskatoon, SK, S7N 5A9, Canada;3. Agriculture and Agri-Food Canada, Saskatoon Research Centre, 107 Science Place, Saskatoon, SK, S7N 0X2, Canada;4. Department of Chemical Engineering, Autonomous University of Yucatan, Calle 60 No. 491-A por 57, Merida, Yucatan 97000, Mexico;5. College of Water Conservancy, Shenyang Agricultural University, 120 Dongling Road, Shenyang, Liaoning, 110866, China;1. Research Institute of Wood Industry of Chinese Academy of Forestry, Hunan Collaborative Innovation Center for Effective Utilizing of Wood & Bamboo Resources, Beijing, 100091, China;2. Center for Renewable Carbon, University of Tennessee, Knoxville, TN 37996, USA;3. Scintillation Materials Research Center, University of Tennessee, Knoxville, TN 37996, USA |
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| Abstract: | Rice straw is the most abundant agricultural residue on a global scale and is widely available as feedstock for cellulosic fuel production. However, it is highly recalcitrant to biochemical deconstruction and also generates inhibitors that affect enzymatic saccharification. Rice straw from eastern Arkansas was subjected to dilute acid pretreatment (160 °C, 48 min and 1.0% sulfuric acid) and solid-state fermentation with two lignocellulolytic fungi, Trametes hirsuta and Myrothecium roridum, and their saccharification efficacies were compared. T. hirsuta and M. roridum were tested separately; pretreatment of rice straw with either strain for seven days resulted in 19 and 70% enrichment of its holocellulose content, respectively. However, liquid chromatography analysis of the alkali extracts showed significant differences in cell wall degradation by T. hirsuta and M. roridum. T. hirsuta removed 15% more phenolic compounds and 38% more glucan than M. roridum, while M. roridum removed 77% more xylan than T. hirsuta. Fungal and dilute acid pretreated biomass was then hydrolyzed using Accellerase® 1500, a saccharification cocktail. Saccharification efficiency of M. roridum was 37% higher than that of dilute acid pretreatment of rice straw, requiring 8% lower enzyme loading and 50% shorter enzymatic hydrolysis duration. Alkali extraction of fungal pretreated biomass also yielded 10–15 g kg?1 of acid precipitable polymeric lignin (APPL), which is a valuable co-product for biorefineries. In comparison to dilute acid pretreatment, fungal pretreatment could be a cost-effective alternative for the degradation of recalcitrant biomass, such as rice straw. |
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| Keywords: | Enzymatic hydrolysis Fungi pretreatment Saccharification Rice straw Dilute acid |
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