反硝化滤池反冲洗效能综合影响因素及微生物种群

采用正交实验考察了水冲洗强度、水单独漂洗时间、气水联合冲洗时间、气冲洗强度、滤料粒径对反硝化滤池反冲洗效能及微生物种群的综合影响。结果表明,当以反硝化滤池运行周期、耗水量、系统恢复时间作为反冲洗效能的综合评价指标时,各因素对综合评价指标影响的显著性次序为:水冲洗强度>气水联合冲洗时间>气冲洗强度>滤料粒径>水单独漂洗时间;得出气水联合反冲洗最优工况参数如下:滤料粒径为2~4 mm、气冲洗强度为15 L/(m²·s)、水冲洗强度为10 L/(m²·s)、气水联合冲洗时间为8 min。PCR-DGGE分析结果表明,反冲洗对滤料层生物量、生物种群多样性有显著影响,对滤料层生物活性、微生物种群丰度影响不显著。

Comprehensive Influencing Factors of Backwashing Efficiency of Denitrifying Biofilter and Its Microbial Community

Effects of water washing intensity,water washing time,gas and water combined washing time, gas washing intensity, filler particle size on backwashing efficiency and microbial community of denitrifying biofilter were investigated by orthogonal experiment. When the operation cycle,water consumption and system recovery time of the denitrifying biofilter were selected as the comprehensive evaluation index of the backwashing efficiency, the significant order of each factor affecting the comprehensive evaluation index was: water washing intensity,gas and water combined washing time,gas washing intensity,filler particle size and water washing time. The optimum conditions were as follows: particle size of 2-4 mm,gas washing intensity of 15 L/( m²·s),water washing intensity of 10 L/( m²·s),and gas and water combined washing time of 8 min. The results of PCRDGGE analysis showed that the backwashing had significant effects on the biomass and biodiversity of the filter layer. However,it had little effect on the biological activity and microbial community abundance of the filter layer.