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催乳素酶促化学发光免疫分析试剂盒的研制
引用本文:贾娟娟,刘一兵,许文革,张雪峰. 催乳素酶促化学发光免疫分析试剂盒的研制[J]. 同位素, 2010, 23(2): 107-110. DOI: 10.7538/tws.2010.23.02.0107
作者姓名:贾娟娟  刘一兵  许文革  张雪峰
作者单位:中国原子能科学研究院,同位素研究所,北京,102413
摘    要:采用两株催乳素(PRL)单克隆抗体,一株包被化学发光板,一株标记辣根过氧化物酶,研制了测定人血清PRL浓度的酶促化学发光免疫分析试剂盒。本试剂盒测定范围50~4 000 mIU/L,灵敏度4.26 mIU/L,批内、批间变异系数分别小于10%、15%,回收率90.3%~100.8%;血清样品倍比稀释后的测定值和稀释度的相关系数为1;本试剂盒正常参考值范围:女性43.2~513.1 mIU/L(n=52),男性54.8~334.3 mIU/L(n=68)。

关 键 词:催乳素  单克隆抗体  化学发光免疫分析

Development of Chemiluminescence Immunoassay Kit for Prolactin
JIA Juan-juan,LIU Yi-bing,XU Wen-ge,ZHANG Xue-feng. Development of Chemiluminescence Immunoassay Kit for Prolactin[J]. Isotopes, 2010, 23(2): 107-110. DOI: 10.7538/tws.2010.23.02.0107
Authors:JIA Juan-juan  LIU Yi-bing  XU Wen-ge  ZHANG Xue-feng
Affiliation:Department of Isotope,China Institute of Atomic Energy, Beijing 102413, China
Abstract:The chemiluminescence immunoassay kit(CLIA) for Prolactin(PRL) in human serum was developed.Two monoclonal antibodies against PRL were applied.One of the antibodies was coated on the microtiter plate,the other was labeled with HRP.The sensitivity of the assay was 4.26 mIU/L and the recovery was 90.3%-100.8%.The intra-and intera-ssay CV were less than 10% and 15% respectively.The results of dilution test demonstrated good correlation between dilution times and values(r=1).The value for normal women samples(n=52) was 43.2-513.1 mIU/L,normal men samples(n=68) was 54.8-334.3 mIU/L.
Keywords:prolactin(PRL)  monoclonal antibody  chemiluminescence immunoassay
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