胰岛素免疫放射分析方法的建立

摘要：采用两株抗胰岛素单克隆抗体，一株包被聚苯乙烯试管作为固相抗体、另一株标记125I，建立了胰岛素测定的双抗体夹心免疫放射分析方法。其方法学灵敏度为2.8mIU/L；曲线范围5～160mIU/L；批内、批间变异系数分别小于5%、10%；回收率92.3％～112.6％；高浓度胰岛素血清样品系列倍比稀释后测定，测定值与稀释度呈线性相关，相关系数0.996；39例健康人血清样品测定值为4.7～14.3mIU/L，该方法与胰岛素放射免疫分析法的相关性良好（r=0.981）。

Establishment of Immunoradiometric Assay for Insulin

Two monoclonal antibodies against insulin are used,one of the antibodies is immobilized to plastic tubes as the solid phase,the other is labelled with 125I as a tracer.The sensitivity of the assay is 2.8 mIU/L and the recovery is 92.3%-112.6%.The intraassay and interassay CVs are below 5% and 10% respectively.The correlation coefficient between measured and expected values is 0.996 after serial dilutions of the samples with high concentration of insulin.The value for normal samples(n=39) is 4.7-14.3 mIU/L.Compared with RIA,the correlation is acceptable(r=0.981).