α粒子与酒精联合作用致肝细胞恶性转化的影响

研究α粒子和酒精联合作用对肝细胞恶性转化生物效应的影响，为系统评价α粒子辐射风险提供实验依据。通过建立辐射和酒精联合作用细胞模型，分析比较α粒子辐射、酒精单因素与两者复合对人正常肝细胞的细胞周期、迁移和侵袭等恶性转化生物特征及相关基因mRNA水平表达的影响。与对照组比较，复合因素作用的细胞发生迁移和侵袭的细胞数量明显增多，在mRNA水平抑癌相关基因p53和细胞粘附蛋白相关基因cdh1表达量明显降低，原癌相关基因mdm2表达量明显升高，复合因素作用下肝细胞发生明显的恶性转化趋势。辐射和酒精均能诱导肝细胞发生恶性转化，肝细胞经α粒子辐照后再受酒精作用其发生恶性转化的风险高于单纯接受α粒子辐照的细胞，酒精增加了α粒子辐照后肝细胞恶性转化风险，可能与影响肝细胞表面E型粘连蛋白的表达有关，导致细胞之间粘连性降低，促进细胞发生迁移和侵袭。因此，在评价α粒子内照射对健康的影响时需要考虑职业人员的饮酒习惯。

Biological Effects of Interal Exposure of α-Particle Interacted With Alcohol on Hepatocytes

In order to comprehensively evaluate the risk of α-particle irradiation, the biological effects of α-particle radiation combined with alcohol on hepatocytes was studied. The testing indicators which were the cell cycle, cell migration and cell invasion, as well as the mRNA expression were compared among the α-particle exposure group, the alcohol group, and the combined factor group. Compared with the control group, the number of migrating and invading cells in the combined group significantly increases, the mRNA expression of the tumor suppressor related genes p53 and cdh1 significantly decreases, and the protooncogene mdm2 significantly increases. Hepatocytes occurres malignant transformation effected with a certain amount of α-particle irradiation and alcohol. But the hepatocytes are more likely to malignant transformation induced by alcohol after the same dose α-particle exposure. Alcohol may play a helper role in inducing malignant transformation. It may be related to the E-cadherin on the surface of hepatocytes, the low expressions of this surface protein, which reduces the adhesion between cells, and promoting cell migration and invasion. Therefore, drinking habits of occupational personnel should be considered in evaluating the health effects of α particle internal irradiation.