| 血清铁生物剂量计的可行性研究 |
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| 引用本文： | 谢丽华,张晓红,胡晓丹,陈峰,张泽群,李群,张海黔.血清铁生物剂量计的可行性研究[J].辐射研究与辐射工艺学报,2016(1):45-50. |
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| 作者姓名： | 谢丽华 张晓红 胡晓丹 陈峰 张泽群 李群 张海黔 |
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| 作者单位： | 南京航空航天大学南京 210016 |
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| 基金项目： | 国防基础科学研究项目(B2520133007)，国家自然科学基金(11575086;31400721)，江苏省自然科学基金(BK20131355)，江苏高校优势学科建设工程项目资助
Supported by National Defense Basic Scientific Research Project(B252013307)，National Nature Science Foundation of China(11575086;31400721)，Nature Science Foundation of Jiangsu Province(BK20131355)，Priority Academic Program Development of Jiangsu Higher Education Institutions |
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| 摘 要： | 研究人体外周血血清经急性γ射线照射后血清铁的浓度变化,探讨其作为生物剂量计的可行性。人体血清经不同剂量γ射线辐照,采用酶联免疫吸附试验(Enzymes linked immunosorbent assay,ELISA)法定量检测血清铁的浓度,建立血清铁浓度与吸收剂量之间的剂量-效应关系。结果显示,血清铁浓度随吸收剂量升高,血清铁浓度与吸收剂量成线性平方关系:男性y=30 676.5+2 383.5x–202.7x2(r=0.99)];女性y=26 974.4+1 611.8x–141.3x2(r=0.98)]。4 Gy剂量时间-效应关系研究发现离体血清铁在2 d内维持稳定。利用建立的线性平方剂量-效应曲线估算的吸收剂量与双盲剂量(1、2、4 Gy)接近,估算值的95%置信区间较窄。研究结果提示血清铁具有重建人体吸收剂量的潜能。
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| 关 键 词： | 生物剂量计 血清铁 γ射线 |
Development of a new biodosimetry of serum iron |
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| Abstract: | The aim is to study the serum iron changesin vitro serum of human peripheral blood irradiated by Co-60γ-rays and explore its feasibility as a biodosimetry. To establish the dose-response relationship, the concentration of serum iron inγ-rays irradiated serum was detected using ELISA (Enzymes linked immunosorbent assay). The results demonstrated that serum iron level increased with absorbed dose, and the dose-response curve of serum iron concentration followed the linear-quadratic model: male y=30 676.5+2 383.5x–202.7x2 (r=0.99)], female y=26 974.4+ 1 611.8x–141.3x2 (r=0.98)]. Time-response relationship of 4 Gy demonstrated that the serum iron was stable within 2 d. With the dose-response linear-quadratic curve, the absorbed dose was close to the double-blind dose (1, 2 and 4 Gy), and the 95% confidence interval of the estimated value was narrow. Therefore, the serum iron has the potential to be a new biodosimetry to reconstruct human exposure dose. |
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| Keywords: | Biological dosimeter Serum iron γ-rays |
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