| 阿魏菇多糖高产菌株筛选的离子束诱变和复合诱变对比研究 |
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| 引用本文： | 王连峰,陈恒雷,张军,曾宪贤. 阿魏菇多糖高产菌株筛选的离子束诱变和复合诱变对比研究[J]. 辐射研究与辐射工艺学报, 2009, 27(4): 248-252 |
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| 作者姓名： | 王连峰 陈恒雷 张军 曾宪贤 |
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| 作者单位： | 新疆大学物理学院离子束生物工程中心,乌鲁木齐,830008 |
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| 基金项目： | 新疆大学青年教师科研启动基金，国家发改委高技术产业化示范工程项目 |
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| 摘 要： | 为筛选出阿魏菇菌丝体多糖高产菌株，本文进行了阿魏菇多糖高产菌株筛选的离子束诱变和复合诱变对比研究。尝试用打散的阿魏菇菌丝体为靶材，以离子束注入为诱变手段，通过营养缺陷型初筛和摇瓶发酵复筛，获得了2株阿魏菇多糖高产菌株PFPH-1和PFPH-2。其菌丝体多糖产量分别达到551．80mg，L和659．46mg／L，比出发菌株分别提高了46．55％和75．14％。再分别以PFPH．1和PFPH．2为出发菌株，用紫外线和氯化锂进行复合诱变处理，通过高通量初筛和摇瓶发酵复筛，结果发现，菌株1、9和10号比其出发菌株PFPH-1的多糖产量分别减少了27％、38％和37％；菌株17号比其出发菌株PFPH-2的多糖产量减少了28％。在本研究中，紫外线和氯化锂的复合诱变表现出了一定的负效应。
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| 关 键 词： | 阿魏菇 多糖 复合诱变 比较 |
The comparative study on screening of pleurotus polysaccharide high-yield strains by use of ion beam implantation and composite mutagenesis |
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| WANG Lianfeng,CHEN Henglei,ZHANG Jun,ZENG Xianxian. The comparative study on screening of pleurotus polysaccharide high-yield strains by use of ion beam implantation and composite mutagenesis[J]. Journal of Radiation Research and Radiation Processing, 2009, 27(4): 248-252 |
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| Authors: | WANG Lianfeng CHEN Henglei ZHANG Jun ZENG Xianxian |
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| Affiliation: | Ion Beam Bio-engineering Center;School of Physics Science and Technology;Xinjiang University;Urumqi 830008;China |
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| Abstract: | In order to screen pleurotus mycelium polysaccharide high-yield strains,the comparative study was made by use of ion beam implantation and composite mutagenesis before screening.The treating mycelium pellet of pleurotus ferulae tentatively with ion beam implantation was performed at the first.Two polysaccharide high-yield strains,PFPH-1and PFPH-2,were selected using fermentation quantitative screening after auxotrophy qualitative primary screening.It has been found that the polysaccharide yield of the mutan... |
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| Keywords: | Pleurotus polysaccharide high-yield strains Ion beam Composite mutagenesis |
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