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LDR辐射后脾细胞裂解成分对淋巴细胞NK活性的影响
引用本文:吕兑财,苏燎原.LDR辐射后脾细胞裂解成分对淋巴细胞NK活性的影响[J].辐射研究与辐射工艺学报,2003,21(3):218-221.
作者姓名:吕兑财  苏燎原
作者单位:1. 航天医学工程研究所,北京,100094
2. 苏州大学放射医学与公共卫生学院医学放射生物学教研究室,苏州,215007
摘    要:研究了低剂量辐射(LDR)后脾细胞裂解成分对淋巴细胞自然杀伤细胞(NK)活性的影响。用McAb直接法分离出CD57细胞和非CD57细胞(除CD57外的所有淋巴细胞)并以两者为效应细胞,K562细胞为靶细胞,将CD57与非CD57杀伤肿瘤细胞的作用进行比较;测定计算各自的杀伤活性并观察LDR辐射后脾细胞裂解成分对杀伤活性的影响。结果表明,CD57及非CD57细胞均对肿瘤细胞有杀伤作用,且非CD57细胞的作用更强;LDR辐射后脾细胞裂解成分能分别增强CD57及非CD57细胞体外抗肿瘤细胞效应(NK细胞活性及NK活性)。

关 键 词:低剂量辐射  脾细胞裂解  淋巴细胞  NK活性  肿瘤细胞  自然杀伤细胞
修稿时间:2002年5月29日

THE EFFECT OF LYSATE OF SPLEEN CELLS AFTER LOW DOSE RADIATION ( LDR ) ON NK ACTIVITY
LU Duicai SU Liaoyuan.THE EFFECT OF LYSATE OF SPLEEN CELLS AFTER LOW DOSE RADIATION ( LDR ) ON NK ACTIVITY[J].Journal of Radiation Research and Radiation Processing,2003,21(3):218-221.
Authors:LU Duicai SU Liaoyuan
Institution:LU Duicai SU Liaoyuan 1
Abstract:To find effect of lysate of spleen cells after LDR on NK activity of CD57 cells or non-CD57 cells. Lysate of spleen cells after LDR were extracted. McAb (anti CD57 cells) was used to separate CD57 cells from human peripheral blood by Panning direct method. The CD57 cells and non-CD57 cells were used as effective cells.K562 cells labeled by 3H-TdR were used as target cells. The ratio of effect cells to target cells was 10:1.NK activity of CD57 cells or non-CD57 cells with the lysate of spleen cells after LDR was reflected by the efficiency of anti tumor cells. The 3H-TdR incorporation in K562 cells cultured with non-CD57 cells was significantly lower than that with CD57 cells. After use of the lysate of spleen cells after LDR, NK activities of NK cells and non-NK cells were 1.24 and 1.58 respectively. They were both increased obviously compared with control groups. The effect of anti K562 cells of non-NK cells is even greater than that of NK cells. The lysate of spleen cells after LDR can enhance the effect of both non-NK cells and NK cells.
Keywords:Low dose radiation (LDR) Lysate of spleen celis  Lymphocyte  NK activity  
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