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| X射线致人外周血线粒体DNA 4977bp缺失的时效关系研究 | |
| 引用本文： | 王金合,王平,姜峰,韩林,王喜爱,吕玉民.X射线致人外周血线粒体DNA 4977bp缺失的时效关系研究[J].辐射防护,2011,31(5). |
| 作者姓名： | 王金合 王平 姜峰 韩林 王喜爱 吕玉民 |
| 作者单位： | 1. 河南省职业病防治研究院,郑州,450052 2. 郑州大学公共卫生学院,郑州,450001 3. 河南省职业病防治研究院,郑州450052／郑州大学公共卫生学院,郑州450001 |
| 基金项目： | 河南省医学科技攻关创新项目(2005077) |
| 摘 要： | 采集2名25岁健康男性外周血进行不同剂量（0～10 Gy）单次X射线照射,于照后取不同时间点（2、12、24、48和72 h）培养的细胞提取基因组DNA,利用实时定量PCR检测不同时间点线粒体DNA（mtDNA）4977bp缺失情况,探讨X射线致人外周血mtDNA 4977bp缺失的时间效应关系。结果表明,受照剂量为5 Gy时,随着照射后培养时间的增加,mtDNA 4977bp缺失拷贝数、mtDNA总拷贝数和mtDNA 4977bp缺失率均有增加趋势。培养时间为24 h和72 h时,在0～10 Gy剂量范围内mtDNA 4977bp缺失拷贝数、mtDNA总拷贝数和mtDNA 4977bp缺失率随着受照剂量的增加而增加。提示X射线诱发的mtDNA 4977bp缺失和mtDNA总拷贝数具有时间和剂量累积性。 |
| 关 键 词： | X射线 线粒体DNA 4977bp缺失 时效关系 |
Time-effect Relationship of Mitochondrial DNA 4977bp Deletion in Human Peripheral Blood Cell after X Ray Irradiation |
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| Wang Jinhe,Wang Ping,Jiang Feng,Han Lin,Wang Xiai,Lu Yumin.Time-effect Relationship of Mitochondrial DNA 4977bp Deletion in Human Peripheral Blood Cell after X Ray Irradiation[J].Radiation Protection,2011,31(5). | |
| Authors: | Wang Jinhe Wang Ping Jiang Feng Han Lin Wang Xiai Lu Yumin |
| Affiliation: | Wang Jinhe1,Wang Ping1,Jiang Feng2,Han Lin1,Wang Xiai1,Lu Yumin1,2(1.Henan Institute of Occupational Medicine,Zhengzhou 450052,2.College of Public Health,Zhengzhou University,Zhengzhou 450001) |
| Abstract: | To investigate the time-effect of mitochondrial DNA 4977bp deletion in human peripheral blood cells exposed to X ray,human peripheral whole blood samples were collected from two healthy individuals,and exposed to X rays with dose from 0 to 10 Gy.The genomic DNAs were isolated from the whole-blood samples,and the levels of mtDNA 4977bp deletion and copy number of total mtDNA in the DNA samples were detected by Real-time PCR after irradiation at 2,12,24,48,and 72 h, respectively.The results showed that the co... |
| Keywords: | X Rays Mitochondrial DNA 4977bp Deletion Time-effect Relationship |
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