固定化细胞提高热带假丝酵母菌的噻吩耐受性及降解效率

利用固定化细胞技术提高热带假丝酵母降解高浓度噻吩的效率,考察了固定化包埋的载体类型及固定化的方法,优化了固定化微球降解噻吩的实验条件。实验结果表明:用2%的海藻酸钠固定化热带假丝酵母,微球表面可形成一层致密的薄膜,将大量细胞包埋在微球里,菌体密度达到5×1010 CFU/g微球;噻吩降解实验中较佳条件为:微球接种量7 g/100 mL、培养基初始pH 8.5~9.0、培养温度30℃。在500 mg/L噻吩的培养液中,培养16 h时降解可达62.3%。

IMMOBILIZED CANDIDA TROPICALIS IMPROVED THE TOLERANCE AND DEGRADATION EFFICIENCY OF THIOPHENE

The degradation rate of high concentration thiophene by Candida tropicalis was improved by immobilized cell technology.The immobilized gel types and immobilized methods were investigated,and the experimental conditions for degradation of thiophene by immobilized microspheres were optimized.The results showed that when 2%sodium alginate gel was used to immobilize Candida tropicalis,a dense film could be formed on the surface of the microspheres.A large number of cells were embedded in the microspheres,and the density of microspheres reached 5×1010 CFU/g.The optimal conditions in the thiophene degradation experiment were that the inoculation volume of microspheres was 7 g/100 mL,the initial pH of the medium was 8.59.0,and the culture temperature was 30℃.In the medium with 500 mg/L of thiophene,the degradation rate reached 62.3%for 16 h culture.