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Genetic identification of global commercial sea cucumber species on the basis of mitochondrial DNA sequences
Authors:Jing Wen  Chaoqun Hu  Lvping Zhang  Sigang Fan
Affiliation:1. Key Laboratory of Marine Bio-resources Sustainable Utilization (LMB), Key Laboratory of Applied Marine Biology of Guangdong Province and The Chinese Academy of Sciences (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, China;2. Department of Biology, Zhanjiang Normal University, Zhanjiang, 524048, China;1. National Marine Science Centre, Southern Cross University, PO Box 4321, Coffs Harbour NSW 2450, Australia;2. School of Marine and Tropical Biology, James Cook University, Townsville 4811 QLD, Australia;3. The WorldFish Center, PO Box 500 GPO, 10670 Penang, Malaysia;4. ARC Centre of Excellence for Coral Reef Studies, James Cook University, Townsville 4811 QLD, Australia;1. Guangdong Provincial Key Laboratory of Biotechnology Candidate Drug Research, School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, PR China;2. CAS Key Laboratory of Tropical Marine Bio-resources and Ecology (LMB), Guangdong Provincial Key Laboratory of Applied Marine Biology (LAMB), South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, 510301, PR China;3. College of Marine Sciences, South China Agricultural University, Guangzhou, 510642, PR China;1. Chulabhorn International College of Medicine, Thammasat University, Rangsit Campus, Pathumthani, 12121, Thailand;2. Division of Health and Applied Sciences, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand;3. GeneCology Research Centre, University of the Sunshine Coast, Maroochydore, Queensland, 4558, Australia;4. Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Maroochydore, Queensland, 4558, Australia;5. Department of Anatomy, Faculty of Science, Mahidol University, Rama VI Road, Ratchathewi, Bangkok, 10400, Thailand
Abstract:Sea cucumbers are regarded as traditional delicacies, medicine and aphrodisiacs in Asia over many centuries. Generally, sea cucumbers are gutted, boiled and roasted, then preserved through drying, smoking or freezing. Thus, it is very difficult to identify clearly the species of processed sea cucumber on the basis of their morphology. In this work, FINS methodology was developed as a tool to assess the incidence of incorrect labeling of sea cucumbers belonging to family Holothuriidae in commercial food products. The result showed that 7 samples were incorrectly labeled (63.6%). Moreover, the technique allows the genetic identification of more than 40 species from all over the world.
Keywords:
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