Analysis of trace methylene blue in fish muscles using ultra-sensitive surface-enhanced Raman spectroscopy |
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| Affiliation: | 1. College of Food Science and Technology, Shanghai Ocean University, No. 999 Hucheng Huan Road, LinGang New City, Shanghai, 201306, China;2. Chifeng Institute for Drug Control, Tianyi Road, New City, Chifeng, Inner Mongolia, 024000, China;3. School of Food Science, Washington State University, Pullman, WA, 99165, USA;1. Promising Centre for Sensors and Electronic Devices (PCSED), Advanced Materials and Nano-Research Centre, Najran University, P.O. Box: 1988, Najran 11001, Saudi Arabia;2. Nanostructured Materials and Nanotechnology Division, Central Metallurgical Research and Development Institute (CMRDI), P.O. Box: 87 Helwan, Cairo 11421, Egypt;3. College of Science and Arts-Sharoura, Najran University, Saudi Arabia;4. Department of Physics, College of Science and Arts, Najran University, P.O. Box 1988, Najran 11001, Saudi Arabia;1. Department of Materials Science and Engineering, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan;2. Center for Micro/Nano Science and Technology, National Cheng Kung University, 1 University Road, Tainan 70101, Taiwan;1. Thermodynamic Division, Istituto Nazionale di Ricerca Metrologica, Strada delle Cacce, 91 10135 Torino, Italy;2. C.Re.A.A. – National Reference Centre for the Surveillance and Monitoring of Animal Feed, c/o Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e valle D’Aosta, via Bologna 148, 10154 Torino, Italy;1. Department of Physics, School of Physical Sciences, Sikkim University, Gangtok 737102, India;2. School of Physics, University of Hyderabad, Hyderabad 500046, India;3. Department of Metallurgical Engineering, IIT (BHU), Varanasi 221005, India |
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| Abstract: | Surface-enhanced Raman spectroscopy (SERS) is an emerging technology with great potential for sensitive and rapid analysis of trace methylene blue (MB) in fish muscle. Five different SERS substrates including two commercial nanostructured surface substrates and three types of Au nanoparticles (NPs) with different diameters were compared to optimize enhancement effects. The highest sensitivity with the lowest detection limit of 5 ng/mL for MB standard solutions and 10 ng/g for MB in fish muscles was achieved using the 18 ± 2 nm Au NPs. Partial least squares regression models developed for quantitative analysis showed high linear correlation between the actual and predicted values for MB (R2 cv = 0.958, RPD = 4.84, RMSE = 6.97 ng/g) in fish muscle. Since Au NPs are easy to prepare, stable and affordable, the high sensitivity and predictability of this method has the potential to be widely used for trace analysis of prohibited drug residues in fish and potentially other foods or biological samples. |
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| Keywords: | Surface-enhanced Raman spectroscopy Methylene blue Gold nanoparticle Rapid analysis Quantitative detection |
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