Fiber optic and light scattering sensors: Complimentary approaches to rapid detection of Salmonella enterica in food samples |
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| Affiliation: | 1. Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA;2. Food Safety Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN, USA;3. Food Hygiene Department, Assiut University, Egypt;4. Department of Comparative Pathobiology, Purdue University, West Lafayette, IN, USA;1. Bayer Animal Health GmbH, Leverkusen, Germany;2. Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Ghent, Belgium;3. Animal Health Care Flanders, Torhout, Belgium;1. Tianjin Key Laboratory of Optoelectronic Detection Technology and System, School of Electronics and Information Engineering, Tiangong University, Tianjin 300387, China;2. Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China;3. Key Laboratory of Opto-Electronics Information Technology (Ministry of Education), School of Precision Instruments and Opto-Electronic Engineering, Tianjin University, Tianjin 300072, China;1. Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei, 230031, PR China;2. School of Safety Engineering, Xi’an University of Science and Technology, Xi’an, 710054, PR China;3. Senboll biotechnology Co. Ltd, Shenzhen, 518118, PR China;4. Institue of Photonics and Quantum Science, Heriot-Watt University, Edinburgh, UK;1. Department of Nano-Bio Energy, Chung-Ang University, Seoul 156-756, Republic of Korea;2. School of Integrative Engineering, Chung-Ang University, Seoul 156-756, Republic of Korea |
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| Abstract: | Salmonella-related foodborne infections present a major public health problem worldwide despite more stringent regulations. Salmonella enterica serovars Enteritidis and Typhimurium are the two most frequent causes of poultry related outbreaks; therefore, their rapid and accurate detection would improve Salmonella control at the farm, processing plant, and at retail. In this study, we investigated if a fiber optic immunosensor and light scattering sensor, BARDOT (bacterial rapid detection using optical scattering technology) could facilitate the detection of these two serovars in naturally contaminated poultry (n = 50). The fiber optic sensor with a detection limit of 103 CFU/ml identified S. enterica in selective enrichment broth in less than 12 h. The colonies (1.0 ± 0.2 mm) produced by plating the enriched samples on selective XLT4 agar for 13–15 h were scanned using BARDOT and S. enterica was identified after matching individual colony scatter patterns to the scatter image library with a sample-to-answer time of about 24 h. Both sensors identified 4 positive samples (8%), which corresponded to the results of the USDA-FSIS protocol, PCR, and lateral flow immunoassays. The colony scatter patterns identified all natural isolates as S. Enteritidis, which was further verified by serovar-specific PCR. The sensors used individually or in combination demonstrate potential for accurate and rapid detection of S. enterica in poultry. |
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| Keywords: | Fiber optic sensor Light scattering sensor BARDOT Food samples PCR Poultry carcass |
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