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A reliable and sensitive time-resolved fluorescent immunochromatographic assay (TRFICA) for ochratoxin A in agro-products |
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| Affiliation: | 1. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan, 430062, PR China;2. Department of Food Science and Technology, College of Agriculture, Isfahan University of Technology, Isfahan, 84156-83111, Iran;3. Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan, 430062, PR China;4. Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan, 430062, PR China;5. Quality Inspection and Test Center for Oilseeds Products, Ministry of Agriculture, Wuhan, 430062, PR China;1. Grain and Oil Food Processing Key Laboratory of Jilin Province, Jilin Business and Technology College, Changchun 130507, China;2. College of Animal Sciences/ College of Life Science, Yangtze University, Jingzhou 434023, China;3. Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China;4. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China;5. Laboratory of Quality & Safety Risk Assessment for Oilseed Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China;6. Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China;7. Quality Inspection & Test Center for Oilseed Products, Ministry of Agriculture, Wuhan, 430062, China;1. State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang 330047, PR China;2. Jiangxi-OAI Joint Research Institute, Nanchang University, 235 Nanjing East Road, Nanchang 330047, PR China;1. College of Food Science and Engineering, Hainan University, 58 Renmin Avenue, Haikou, 570228, PR China;2. Department of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California, Davis, CA, 95616, United States;3. State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing East Road, Nanchang, 330047, PR China;1. College of Traditional Chinese Medicine, Jilin Agricultural University, Changchun 130118, China;2. Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, China;3. Pharmacy College, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China;1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. Jiangxi Province Center for Disease Control and Prevention, Nanchang 330047, China;1. Department of Applied Chemistry, College of Materials and Energy, South China Agricultural University, Guangzhou 510642, China;2. The Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China |
| Abstract: | Immunochromatographic assays (ICAs) are considered as a suitable diagnostic tool for the detection of mycotoxins. Mycotoxins and especially, ochratoxin A are analytes with more demanding sensitivity requirements. To enhance the sensitivity of current immunochromatographic assays for ochratoxin A (OTA), a novel sensitive ICA was developed in this study. In the assay, microspheres enclosing fluorescent europium (III) Eu(III)] nanoparticles (EuNPs) were used as a label for OTA monoclonal antibody (OTA-mAb) conjugation. Accordingly, assay was called time-resolved fluorescent immunochromatographic assay (TRFICA). The test strip was composed of three parts: a sample pad, nitrocellulose membrane and an absorbent pad. As for detection, a proper concentration of conjugated microspheres was pipetted into the microtube and sample extract was added to it. Then the strip was inserted into the tube and the fluid flow along the strip. The TRFICA results were obtained in 8 min and read by a portable TRFICA strip reader. The established method allows quantitative determination of OTA with limit of detection as low as 1.0 μg kg?1 in the samples. For validation, spiked samples including wheat, maize, soybean and rice were respectively assayed by TRFICA and a standard high performance liquid chromatography equipped with a fluorescence detector (HPLC-FLD), and good agreement of results was obtained between two methods. |
| Keywords: | Time-resolved fluorescent immunochromatographic assay (TRFICA) Ochratoxin A Agro-products Carboxylate-modified Eu (III)-polystyrene nanoparticles ICAs"} {"#name":"keyword" "$":{"id":"kwrd0035"} "$$":[{"#name":"text" "_":"Immunochromatographic assays OTA"} {"#name":"keyword" "$":{"id":"kwrd0045"} "$$":[{"#name":"text" "_":"Ochratoxin A EuNPs"} {"#name":"keyword" "$":{"id":"kwrd0055"} "$$":[{"#name":"text" "_":"Europium (III) [Eu(III)] nanoparticles EuNPs–Ab"} {"#name":"keyword" "$":{"id":"kwrd0065"} "$$":[{"#name":"text" "_":"Europium (III) nanoparticles labeled antibodies OTA-mAb"} {"#name":"keyword" "$":{"id":"kwrd0075"} "$$":[{"#name":"text" "_":"OTA monoclonal antibody TRFICA"} {"#name":"keyword" "$":{"id":"kwrd0085"} "$$":[{"#name":"text" "_":"Time-resolved fluorescent immunochromatographic assay HPLC-FLD"} {"#name":"keyword" "$":{"id":"kwrd0095"} "$$":[{"#name":"text" "_":"High performance liquid chromatography equipped with a fluorescence detector OVA"} {"#name":"keyword" "$":{"id":"kwrd0105"} "$$":[{"#name":"text" "_":"Ovalbumin BSA"} {"#name":"keyword" "$":{"id":"kwrd0115"} "$$":[{"#name":"text" "_":"Bovine serum albumin LOD"} {"#name":"keyword" "$":{"id":"kwrd0125"} "$$":[{"#name":"text" "_":"Limit of detection PBS"} {"#name":"keyword" "$":{"id":"kwrd0135"} "$$":[{"#name":"text" "_":"Phosphate buffer solution BBS"} {"#name":"keyword" "$":{"id":"kwrd0145"} "$$":[{"#name":"text" "_":"Borate buffer solution GNPs"} {"#name":"keyword" "$":{"id":"kwrd0155"} "$$":[{"#name":"text" "_":"Gold nanoparticles |
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