| 猪源金黄色葡萄球菌vwb基因分布及对不同动物体外凝血致病作用的研究 |
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| 引用本文： | 张阳,闫鹤.猪源金黄色葡萄球菌vwb基因分布及对不同动物体外凝血致病作用的研究[J].现代食品科技,2018,34(9):88-94. |
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| 作者姓名： | 张阳 闫鹤 |
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| 作者单位： | 华南理工大学食品科学与工程学院 |
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| 基金项目： | “十三五”国家重点研发计划项目（2016YFD0500606）；广东省科技计划项目（2014A020214001；2016A020219001）；中央高校建设世界一流大学（学科）和特色发展引导专项资金（K5174960）；中央高校基本科研业务费（D2170320） |
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| 摘 要： | 为调查猪源金黄色葡萄球菌(Staphylococcus aurues,S. aureus)对不同动物宿主具有凝血致病作用及是否携带S. aureus凝固酶基因vwb~C、vwb~Sbo4、vwb~Sov2和vwb~Seq1,本文以厦门地区生猪养殖场、屠宰场和终端市场分离的130株S. aureus为研究对象,通过血浆凝固酶实验研究菌株对兔血浆、牛血浆和羊血浆的凝血致病性,采用PCR方法对实验菌株进行vwb~C、vwb~(Sbo4)、vwb~(Sov2)和vwb~(Seq1)基因的扩增。结果表明,兔血浆、牛血浆和羊血浆的阳性凝血率分别为100.00%、28.46%、28.46%。S.aureus凝固酶vwb~C,vwb~(Sbo4)和vwb~(Sov2)基因的检出率分别为100.00%(130/130),26.92%(35/130)和26.93%(38/130),vwb~(Seq1)基因未检出;来自生猪养殖场S.aureus中vwb~(Sbo4)和vwb~(Sov2)基因的检出率以及对牛血浆和羊血浆的阳性凝血率明显高于另外两种来源S. aureus,有显著性差异(p0.05)。研究结果说明猪源S. aureus对不同动物宿主具有凝血致病作用,为猪源S. aureus对不同动物宿主的凝血致病作用和适应性提供研究基础。
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| 关 键 词： | 金黄色葡萄球菌 凝固酶基因 凝血致病性 |
| 收稿时间： | 2018/1/9 0:00:00 |
The Distribution of vwb genes and Coagulation Pathogenicity to Different Animal Hosts in Porcine Staphylococcus aureus |
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| ZHANG Yang and YAN He.The Distribution of vwb genes and Coagulation Pathogenicity to Different Animal Hosts in Porcine Staphylococcus aureus[J].Modern Food Science & Technology,2018,34(9):88-94. |
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| Authors: | ZHANG Yang and YAN He |
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| Affiliation: | (School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China) and (School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China) |
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| Abstract: | In this work, to investigate the coagulation pathogenicity of porcine Staphylococcus aureus to different animal hosts and whether they harbor staphylococcal coagulase genes of vwbC, vwbSbo4, vwbSov2 and vwbSeq1, 130 S. aureus were isolated from swine farm, slaughterhouse and terminal market in Xiamen. Coagulation adaptability of these isolates to rabbit, bovine and goat plasma were studied by coagulase assays. The distribution of vwbC, vwbSbo4, vwbSov2 and vwbSeq1 genes in 130 S. aureus were also investigated by PCR amplification. The results showed that positive coagulation rate of 130 S. aureus to rabbit, bovine and goat plasma were 100.00%, 28.46% and 28.46%, respectively. The staphylococcal coagulasegenes of vwbC, vwbSbo4 and vwbSov2 were identified in 100.00% (130/130), 26.92% (35/130) and 26.93% (38/130) of all isolates, respectively. None of isolates harbored vwbSeq1gene.The detection rates ofvwbSbo4 and vwbSov2 genes and the positive coagulation rates of bovine and goat plasma of S. aureus isolated from pig farms were significantly higher than those isolated from slaughterhouse and terminal marker isolates (p<0.05). This work showed that porcine S. aureus possessed coagulation pathogenicity to different animal hosts and provided a basis for studying on the coagulation pathogenicity and host adaptability of porcine S. aureus. |
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| Keywords: | Staphylococcus aureus coagulation genes coagulation adaptability |
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