| 三唑磷多克隆抗体的制备及鉴定 |
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| 引用本文： | 黄魁英,雷红涛,孙远明.三唑磷多克隆抗体的制备及鉴定[J].现代食品科技,2011,27(12):1469-1471. |
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| 作者姓名： | 黄魁英 雷红涛 孙远明 |
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| 作者单位： | 1. 华南农业大学食品学院广东省食品质量安全重点实验室,广东广州510642;广州市微生物研究所,广东广州510663
2. 华南农业大学食品学院广东省食品质量安全重点实验室,广东广州,510642 |
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| 摘 要： | 采用活泼酯法将三唑磷半抗原(TZPM-Hap)与牛血清蛋白(BSA)和卵血清蛋白(OVA)偶联,制备出免疫抗原(TZPM-A-BSA)和包被抗原(TZPM-A-OVA),经紫外扫描证明人工抗原合成成功.用免疫抗原TZPM-A-BSA免疫雌性Balb/c小鼠,获得了特异性的三唑磷多克隆抗体.以所获得的抗体建立间接竞争酶联...
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| 关 键 词： | 三唑磷 活泼酯法 人工抗原 多克隆抗体 酶联免疫吸附检测 |
Production and Identification of Polyclonal Antibody against Triazophos |
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| HUANG Kui-ying,LEI Hong-tao,SUN Yuan-ming.Production and Identification of Polyclonal Antibody against Triazophos[J].Modern Food Science & Technology,2011,27(12):1469-1471. |
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| Authors: | HUANG Kui-ying LEI Hong-tao SUN Yuan-ming |
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| Affiliation: | 1(1.Key Laboratory of Food Quality and Safety of Guangdong Province,College of Food Science,South China Agricultural University,Guangzhou 510642,China)(2.Guangdong Institute of Microbiology,Guangzhou 510663,China) |
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| Abstract: | In this paper,artificial antigens for triazophos including one immunogen and one coating antigen were prepared.The hapten(TZPM-Hap) of triazophos was coupled to bovine serum albumin(BSA) and ovalbumin(OVA) by the active ester method to prepare the artificial immunogen TZPM-A-BSA and the coating antigen TZPM-A-OVA,respectively.Immunogen and coating antigen were successfully prepared and confirmed by ultraviolet spectrometry.Polyclonal antibodies against triazophos were produced from female Balb/c mice,which were immunized with the artificial immunogen TZPM-A-BSA.Indirectly competitive ELISA was established with this antiserum,of which IC50 was 0.01 μg/mL.The polyclonal antibodies showed no obvious cross-reactivity with other structural-related organophosphate pesticides. |
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| Keywords: | triazophos active ester method artificial antigen polyclonal antibody enzyme-linked immunosorbnent assay |
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