胶体金免疫层析技术快速检测沙丁胺醇残留

建立胶体金免疫层析检测法,用于动物组织或饲料样品中沙丁胺醇残留的快速检测。采用胶体金免疫竞争法,将兔抗沙丁胺醇多克隆抗体-胶体金标记物包被在胶体金聚酯结合垫上,并将沙丁胺醇-BSA抗原包被在硝酸纤维素膜上作为检测线,与待测试样中的沙丁胺醇竞争结合胶体金标记的兔抗沙丁胺醇多克隆抗体,从而判断试样中是否有沙丁胺醇存在。本文优化了检测试剂,可检测出猪肉等组织和饲料试样中沙丁胺醇的低限浓度为2 ng/mL,与盐酸克伦特罗的交叉反应率为2.67%,且试验在3 min内完成,肉眼可判断结果。本检测方法检测沙丁胺醇,灵敏度高,特异性好,可用于现场的快速检测和筛查。

Colloidal Gold Immunochromatographic Assay for Residual Sabutamol

Colloidal gold immunochromatography assay can rapidly test residual subutamol in meat and animal feed. An anti-salbutamol antibody is conjugated to colloidal gold and placed on conjugate pad. Salbutamol-BSA antigen is coated on nitrocellulose membrane as test line (T line). Colloidal gold provides color to visualize antibody-antigen binding. If there is Salbutamol in the sample, it will bind gold-antibody conjugate and prevent its binding onto the Salbutamol-BSA antigen in the test line(T line) on membrane. As result, no color will be visible on test line on membrane. Otherwise, anti-salbutamol antibody conjugated to colloidal gold will bind the Salbutamol-BSA antigen leading to red color on test line. The color of test line indicates positive or negative result. With optimized reagents, the lowest limit of detection is 2ng/ml. The cross reaction with Clenbuterol is 2.67%. Results show in 3 minutes and are visible to naked eyes. This method provides a sensitive and specific detection approach for the presence of salbutamol in meat and animal feed on site.