| 胆固醇氧化酶高产菌类芽胞杆菌的诱变选育 |
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| 引用本文： | 王发合,杨辉,朱萍,梁海秋,周河治.胆固醇氧化酶高产菌类芽胞杆菌的诱变选育[J].现代食品科技,2007,23(3):7-10. |
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| 作者姓名： | 王发合 杨辉 朱萍 梁海秋 周河治 |
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| 作者单位： | 广西大学生命科学与技术学院,广西,南宁,53004 |
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| 摘 要： | 由本实验室产胆固醇氧化酶的菌株类芽胞杆菌作为出发菌株，经过紫外＋LiCl、紫外＋光敏试剂（8-mop）物理化学诱变处理，最后得到一株突变株XUM-10，并对其进行发酵条件的优化，最终酶活为221．20U／L，其酶活为出发菌株的2.5倍。
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| 关 键 词： | 类芽胞杆菌 胆固醇氧化酶 诱变选育 |
| 文章编号： | 1673-9078(2007)03-0007-04 |
| 收稿时间： | 2006/11/17 0:00:00 |
| 修稿时间： | 2006年11月17 |
Mutation and Breeding of Paenibacillus sp. with high Cholesterol Oxidase Activity |
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| WANG Fa-he,YANG Hui,ZHU Ping,LIANG Hai-qiu,ZHOU He-zhi.Mutation and Breeding of Paenibacillus sp. with high Cholesterol Oxidase Activity[J].Modern Food Science & Technology,2007,23(3):7-10. |
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| Authors: | WANG Fa-he YANG Hui ZHU Ping LIANG Hai-qiu ZHOU He-zhi |
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| Affiliation: | (College of Life Science and Technology, Guangxi University, Nanning 530004,China) |
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| Abstract: | The mutation strain was obtained from Paenibacillus sp. X534 of our lab, and was mutagenized with Ultraviolet LiCl and Ultraviolet 8-mop, giving XUM-10 strain. The fermentation conditions of XUM-10 strain were also studied. Results showed that the enzyme activity was 221.20U/L, which was 2.5 times higher than parent strain. |
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| Keywords: | Paenibacillus sp cholesterol oxidase mutation and breeding |
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