| 荞麦中芦丁的提取工艺优化 |
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| 引用本文： | 黄艳菲,彭镰心,丁玲,李艳丹,左旭,刘圆,赵钢.荞麦中芦丁的提取工艺优化[J].现代食品科技,2012,28(10):1345-1349. |
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| 作者姓名： | 黄艳菲 彭镰心 丁玲 李艳丹 左旭 刘圆 赵钢 |
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| 作者单位： | 西南民族大学民族医药研究院;成都大学生物产业学院 |
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| 基金项目： | 国家科技支撑计划项目(2012BAI27B07);四川省科技支撑计划(2011SZ0233);现代农业产业技术体系建设专项资金资助(CARS-08-D-3);四川中医药管理局课题(2010-78) |
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| 摘 要： | 以米荞1号为原料,采用响应面法优化荞麦中芦丁的提取工艺。在单因素试验的基础上,根据Box-Behnken试验设计原理,通过响应面法优化芦丁提取工艺,选取时间、温度、料液比和甲醇体积分数四因素三水平进行中心组合试验,建立芦丁含量的二次回归方程,确定提取芦丁的最佳工艺组合条件。结果表明:提取时间93 min,提取温度77℃,料液比1:32,甲醇体积分数91%。该条件下芦丁提取含量预测值为17.92 mg/g,实测值为18.20mg/g,偏差不大。
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| 关 键 词： | 荞麦 芦丁 响应面 提取工艺 |
| 收稿时间： | 2012/6/10 0:00:00 |
Optimization of Rutin Extraction from Fagopyrum |
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| HUNAG Yan-Fei,PENG Lian-Xin,DING Ling,LI Yan-dan,ZUO Xu,LIU Yuan and ZHAO Gang.Optimization of Rutin Extraction from Fagopyrum[J].Modern Food Science & Technology,2012,28(10):1345-1349. |
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| Authors: | HUNAG Yan-Fei PENG Lian-Xin DING Ling LI Yan-dan ZUO Xu LIU Yuan and ZHAO Gang |
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| Affiliation: | 1.Ethnic Medicine Institute,Southwest University for Nationalities,Chengdu 610041,China)(2.College of Biotechnology Industries,Chengdu University,Chengdu 610106,China) |
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| Abstract: | The response surface methodology was used to optimize the extraction of Fagopyrum of Miqiao 1.Based on one-factor-at-a-time experiments,the extraction of time,temperature,liquid-to-solid ratio and methanol concentration were used to carry out a 4-variable,3-level central composite design,and established quadratic regression model.The best extraction time,temperature,solid-liquid ratio and methanol content were 93 minute,77 ℃,1:32 and 91 % under which the predicted yield of rutin with the optimized procedure was 17.92 mg/ and the practical yield of rutin was 18.20 mg/g. |
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| Keywords: | buckwheat rutin response surface methodology optimization of extraction |
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