| 益生菌制剂对抗生素诱导腹泻模型小鼠肠道菌群的恢复 |
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| 引用本文： | 金鑫,葛亚中,敬璞,游丽君.益生菌制剂对抗生素诱导腹泻模型小鼠肠道菌群的恢复[J].现代食品科技,2017,33(3):17-24. |
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| 作者姓名： | 金鑫 葛亚中 敬璞 游丽君 |
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| 作者单位： | （1.大连工业大学食品学院，辽宁大连 116034）,（1.大连工业大学食品学院，辽宁大连 116034）,（1.大连工业大学食品学院，辽宁大连 116034）（2.东北农业大学食品安全与营养协同创新中心,黑龙江哈尔滨 150030）,（1.大连工业大学食品学院，辽宁大连 116034）,（1.大连工业大学食品学院，辽宁大连 116034）,（1.大连工业大学食品学院，辽宁大连 116034）,（1.大连工业大学食品学院，辽宁大连 116034） |
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| 基金项目： | 国家十二五科技支撑计划项目（2013BAD18B09）；国家自然科学基金项目（31571813）；国家海洋食品工程技术研究中心项目（2012FU125X03）；辽宁省自然科学基金项目（2014026018）资助 |
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| 摘 要： | 本论文采用肠上皮细胞模型HT-29细胞系研究8株植物乳杆菌在HT-29细胞上的黏附性，并研究黏附性较强的菌株及其胞外多糖（ExopolySaccharides，EPS）抑制大肠杆菌(E?coliATCC25922)在HT-29细胞上黏附及刺激HT-29细胞产生炎性因子的作用。结果表明8株植物乳杆菌在HT-29细胞上的黏附性差异较大：黏附性最强的植物乳杆菌35通过取代、竞争和排阻方式抑制大肠杆菌在HT-29细胞上黏附，抑制黏附率分别为30%、33%和59%，其EPS在作用浓度为500 μg/mL时对大肠杆菌的抑制黏附率为32%。植物乳杆菌35可抑制大肠杆菌刺激HT-29细胞产生IL-8，通过取代、竞争、排阻方式抑制大肠杆菌刺激HT-29细胞产生IL-8，抑制率分别为3%、28%和40%；其EPS抑制大肠杆菌刺激HT-29细胞产生IL-8具有浓度效应，浓度为500 μg/mL时抑制率最高，为50%。而对于IL-10表达量的影响均不显著。结果表明植物乳杆菌35具有抑制大肠杆菌引起肠炎的潜在益生功能。
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| 关 键 词： | 乳酸杆菌 HT-29 大肠杆菌 黏附 |
| 收稿时间： | 2016/1/25 0:00:00 |
The Effects of Probiotics on the Restoration of Intestinal Microflora in a Mouse Model of Antibiotic-induced Diarrhea |
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| JIN Xin,GE Ya-zhong,JING Pu and YOU Li-jun.The Effects of Probiotics on the Restoration of Intestinal Microflora in a Mouse Model of Antibiotic-induced Diarrhea[J].Modern Food Science & Technology,2017,33(3):17-24. |
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| Authors: | JIN Xin GE Ya-zhong JING Pu and YOU Li-jun |
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| Affiliation: | (1.School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China),(1.School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China),(1.School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China)(2.The Innovation Center of Food Safety and Nutrition of Northeast Agricultural University, Harbin 150030, China),(1.School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China),(1.School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China),(1.School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China) and (1.School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, China) |
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| Abstract: | In order to evaluate the effects of probiotics on the restoration of intestinal microflora in a mouse model of diarrhea, 22.4 g/(kg/d) ampicillin was administered by gavage to construct the mouse model of diarrhea, and the blank control group was given saline at the same dosage. After the model was successfully constructed, low-, medium-, and high-dose probiotics were used to treat the mice to restore their intestinal microflora. The effect of probiotics on the loose stool rate, degree of loose stool, and diarrhea index were observed after 14 days of treatment. The aseptically collected fecal samples of mice at days 0, 7, and 14 after treatment were analyzed, and changes in the target intestinal flora were determined using denaturing gradient gel electrophoresis (DGGE) and plate counting methods. After the completion of probiotic treatment, the fecal samples of mice were collected aseptically at days 0, 7, and 14, and real-time fluorescent quantitative PCR (FQ-PCR) was carried out for the quantitative determination of exogenous probiotics. The results showed that probiotics not only mitigated diarrhea in mice and promoted mouse growth, but also exhibited a significant effect on the restoration of intestinal flora in mice with diarrhea. The results from FQ-PCR analysis indicated that the exogenous probiotics Lactobacillus acidophilus NCFM, Bifidobacterium lactis Bi-07, and Lactobacillus rhamnosus HN001 were able to colonize and survive after the administration of probiotics was stopped, but showed a certain downward trend over time. |
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| Keywords: | Lactobacillus HT-29 cells E coli ATCC25922 adhesion |
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