正交试验法优选艾叶总黄酮提取工艺研究

本文为测定艾叶中总黄酮的含量采用,设计正交设计优选艾叶中总黄酮的提取条件,用芦丁作对照品,硝酸铝作显色剂,测定波长在510 nm处,比色法测定艾叶总黄酮的吸光度.实验结果获得优选提取条件是:用70 mL 60 %的乙醇浸泡1.000 g左右的艾叶粉末24 h,在超声波功率为200 W的条件下,辅助萃取40 min.结论:艾叶中含有丰富的黄酮类化合物,具有很强的抗氧化活性,认为艾叶有很强的开发应用价值.

Research on Ultrasonic Wave Extraction of Total Flavones from Folium Artemisiae Argyi

Object:To investigating the optimum extracting conditions of the total flavones in Folium artemisiae argyi.Methods:The extracting conditions of the total flavones in folium artemisiae argyi by ultrasonic waves were optimized by the single-factor and orthogonal experiments.Using rutin as control reagent and aluminium nitrate as colorimetric reagent,the absorbency of total flavones was determined with colorimetric wavelength at 510 nm.Results:The best dipping time in 60% alcohol,ulgtrasonic wave power and extraction time were 24 h,200 W and 40 min,respectively.Conclusions:The flavonoid compounds were in abundance in Folium Artemisiae Argyi and had strong anti-oxidant activity.The results also indicated a promising prospect fro development ofFolium Artemisiae Argyi.