高效萃取体系RP-HPLC测定食品中的对位红和苏丹红

本文研究使用高效萃取体系,建立反相高效液相色谱(RP-HPLC)同时测定食品中对位红、苏丹红Ⅰ～Ⅳ号及苏丹红7B.样品用甲醇-乙腈-丙酮萃取液提取,采用聚硅烷C1s色谱柱(Shiseido C18 150mm×4.6 mm i.d,5μm)分离以水-乙腈为流动相,流速1.0mL/min,等度洗脱;用紫外-可见光二极管阵...

Determination of Para Red and Sudan Red Dyes in Food by Reversed Phase High Performance Liquid Chromatography

A method was developed for the determination of Para Red and Sudan dyes in food by reversed phase-high performance liquid chromatography(RP-HPLC) with a new simple extract system.Sample was extracted from food with methanol-acetonitrile-acetone.The analysis was performed on a Shiseido C18 column(150 mm×4.6mm i.d.,5μm),with water-acetonitrile as the mobile phase at a flow rate of 1.0mL/min.Detection was carried out at the wavelength of 500nm and confirmed by spectra.All of the six compounds demonstrated good linear relationship(r=0.9998).The limit of detection(LOD)(spiked at the levels of 0.5~5.0 μg/mL) was 0.1 mg/kg.The average recoveries for all six dyes were ranged from 85%~106%,and the relative standard deviations were form 2.14%~4.82%.The method is sensitive,reliable and can be applied for the analysis of Para Red and Sudan dyes in food.