| 大肠杆菌9种有机酸代谢产物的高效液相检测条件优化 |
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| 引用本文： | 马建刚,刘娇,曾祥峰,郑平,贾士儒,孙际宾.大肠杆菌9种有机酸代谢产物的高效液相检测条件优化[J].现代食品科技,2011,27(5):591-594,519. |
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| 作者姓名： | 马建刚 刘娇 曾祥峰 郑平 贾士儒 孙际宾 |
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| 作者单位： | 1. 天津科技大学生物工程学院,天津,300457
2. 中国科学院天津工业生物技术研究所,天津,300308 |
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| 基金项目： | 中国科学院知识创新工程重要方向项目，天津市科技支撑计划重点项目 |
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| 摘 要： | 以高交联度磺化苯乙烯-二乙烯基苹共聚物多孔微球作为固定相的Aminex HPX-87H离子色谱柱为分析柱,通过优化条件对E.coli MG1655及其突变株厌氧发酵过程中九种有机酸(琥珀酸、甲酸、乙酸、乳酸、草酰乙酸、丙酮酸、富马酸,乙醛酸、柠檬酸)进行了有效的分离,尤其做到了对富马酸与其他有机酸的较好分离,为代谢过程...
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| 关 键 词： | 大肠杆菌 有机酸 代谢产物 |
Optimization of HPLC determination Conditions for 9 Organic Acids Produced by E.coli |
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| MA Jian-gang,LIU Jiao,ZENG Xiang-feng,ZHENG Ping,JIA Shi-ru,SUN Ji-bin.Optimization of HPLC determination Conditions for 9 Organic Acids Produced by E.coli[J].Modern Food Science & Technology,2011,27(5):591-594,519. |
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| Authors: | MA Jian-gang LIU Jiao ZENG Xiang-feng ZHENG Ping JIA Shi-ru SUN Ji-bin |
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| Affiliation: | 1.School of Biological Engineering,Tianjin University of Science and Technology,Tianjin,300457,China)(2.Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin,300308,China) |
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| Abstract: | Ion chromatographic column Aminex HPX-87H filled with highly cross-linked sulfonated polystyrene divinylbenzene porous microspheres was used to determine 9 kinds of organic acids in the anaerobic fermentation broth of E.coli MG1655 and its mutant.After optimization of HPLC conditions,nine kinds of organic acids(succinic acid,formic acid,acetic acid,lactic acid,oxaloacetate,pyruvate,fumaric acid,glyoxylic acid,citric acid) were successfully separated.In particular,fumaric acid was well separated from the other organic acids,which provides important support for metabolic process monitoring and subsequent strain development.The optimal determination conditions were: mobile phase 2.75 mM H2SO4,flow rate 0.6 mL/min,and column temperature 50 ℃.The recovery of this method was 97.9%~106.7% and RSD was 0.8%~3.3%. |
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| Keywords: | E coli organic acid metabolite |
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