胶体金免疫层析法检测食品中的磺胺类药物残留

建立了一种用于快速检测动物源性食品中五种磺胺类药物,磺胺异噁唑(SIZ)、磺胺噻唑(STZ)、磺胺对甲氧嘧啶(SME),磺胺甲二唑(SMT)、磺胺氯哒嗪(SCPA)残留的胶体金免疫层析方法.采用柠檬酸三钠还原法制备粒径为20nm的胶体金,将对磺胺类药物具有广谱特异性的多克隆抗体-肢体金复合物喷涂在玻璃纤维上,并将人工合...

Detection of Sulfonamides Residues in Food by Colloidal Gold Immunochromatographic Method

A colloidal gold immunochromatography method was established for rapid detection of five kinds of sulfonamides,sulfisozole(SIZ),sulfathiazole(STZ),sulfameter(SME),sulfamethizole(SMT),sulfachloropyrazine(SCPA),from food of animal origin.Tri-sodium citrate with aqueous gold chloride were mixed to make 20 nm colloidal gold which were labeled to the Broad-spectrum specificitical anti-SAs polyclonal antibody,and the compound was sprayed onto the glass fiber.The SAs-OVA and goat anti-rabbit IgG were jet-positioned onto anitrocellulose membrane dispense on test line and control line respectively.Colloidal gold was coupled with monoclonal antibody against sulfonamides served as the test strip,test results can be directly observed by eye measurement.For pork,chicken,carp samples,the visual detection limit was 0.01 mg/kg,and the detection time was less than 10 minutes.This method was fast,simple and reliable,with high sensitivity,specificity.It was proved to be used to spot monitor the sulfonamides residues as an effective means of screening.