酸枣多糖对小鼠CCl4急性肝损伤的作用

本实验提取酸枣多糖对其进行其体外抗氧化活性和治疗肝损伤作用的研究。用95%乙醇浸泡酸枣脱脂,再用蒸馏水浸提酸枣多糖;对酸枣多糖总糖和蛋白质含量进行测定,并探究酸枣多糖清除DPPH、羟自由基的能力;建立小鼠急性CCl4肝损伤模型,给药后分别测定血清中ALT、AST的活性,肝组织匀浆中CAT、SOD的活性以及MDA的含量;通过做肝组织的病理切片来观察酸枣多糖对小鼠肝组织的影响。体外实验结果表明酸枣多糖具有很好的体外抗氧化活性。在体内实验中,实验组与模型组相比,肝组织损伤情况得到明显改善;血清中ALT、AST活性显著下降,最高达34.33%以上;肝组织匀浆中CAT、SOD活性明显升高,最高达到35.88%以上,MDA的含量显著降低,最高达到52.07%;酸枣多糖对小鼠急性CCl4性肝损伤具有明显的治疗作用,其机制可能与调节血清酶、抗氧化物酶的活力和含量有关。

Effect of Polysaccharides from Wild Jujube on Acute Liver Injury Induced by CCl4 in Mice

Polysaccharides from wild jujube (PWJS) were isolated by distilled water extraction and its anti-oxidative activity and liver protection capability were investigated. Acute injury models were set by CCl4 in mice and ALT, AST activities in serum, CAT, SOD activities, MDA content in liver homogenates were tested by biochemical assays. Liver tissue of the mice was stained with hematoxylin-eosin (HE) for observing the effect of PWJS on mice liver histopathology. Results showed that PWJS had strong antioxidant ability in vitro. Meanwhile, in vivo study showed that, compared with model group, ALT and ALP activities in serum declined significantly and the maximal decline rate was 34.33%. CAT, SOD activities in liver homogenates significantly increased, with the maximum increasing rate being 35.88%. MDA content in liver homogenates declined significantly and the maximal decline rate was high to 52.07%. Thus, PWJS successfully reduce hepatotoxicity in CCl4-induced liver injury mice, due to the regulation of serum enzymes and antioxidant enzymes activity.