野艾蒿挥发油的GC-MS分析及其体内抗氧化活性研究

本文研究了野艾蒿挥发油的GC-MS分析及其在小鼠体内的抗氧化活性。采用水蒸气蒸馏法对野艾蒿中的挥发油进行提取,并通过GC-MS对野艾蒿挥发油进行成分分析。结果表明,从野艾蒿中鉴定出8种成分,主要为桉叶脑、酮、醇、烷类等。采用连续六周腹腔注射D-半乳糖的方法构建衰老模型,并测定小鼠心、肝、肾的SOD、GSH、PCO、MDA含量以及对小鼠脏器组织形态进行观察。结果表明,与空白对照组相比,衰老模型组小鼠的心、肝、肾的SOD和GSH含量显著降低(p0.05,p0.01,p0.001),MDA和PCO含量显著升高(p0.001),四个指标以心脏器官影响较大,SOD和GSH含量分别降低了57.72%和44.58%,MDA和PCO含量分别升高了43.59%和86.49%。说明小鼠衰老模型构建成功;与衰老模型组小鼠相比,野艾蒿挥发油保护组小鼠的心、肝、肾的SOD和GSH含量显著升高(p0.05,p0.01,p0.001),MDA和PCO含量显著降低(p0.05,p0.01,p0.001),其中SOD含量对肾脏影响较大,升高了80.42%;GSH和MDA含量对心脏影响较大,分别升高了73.26%,降低了46.93%;PCO含量对肝脏影响较大,降低了47.16%。说明野艾蒿挥发油在小鼠体内具有一定的抗氧化作用。病理切片结果表明,野艾蒿挥发油可以保护氧化损伤小鼠心肌细胞、肝细胞及肾脏细胞形态的完整性。

GC-MS Analysis of Volatile Oil from Artemisia argyi and Its Antioxidant Activity in Vivo

In this paper, the GC-MS analysis of volatile oil from Artemisia argyi and its antioxidant activity in mice were studied. The volatile oil in Artemisia argyi was extracted by steam distillation, and the volatile oil of Artemisia argyi was analyzed by GC-MS. The results showed that eight components were identified from Artemisiaargyi, mainly eucalyptus brain, ketone, alcohol, and alkane. The aging model was established by intraperitoneal injection of D-galactose for six weeks, and the contents of SOD, GSH, PCO and MDA in the heart, liver and kidney of mice were measured and the morphology of mouse organs was observed. The results showed that the SOD and GSH contents in the heart, liver and kidney of the aging model group were significantly lower than those in the blank control group (p<0.05, p<0.01, p<0.001), and the MDA and PCO contents were significantly increased (p<0.001), the four indicators were much more affected in cardiac organs, SOD and GSH levels were reduced by 57.72% and 44.58%, respectively, and MDA and PCO levels were increased by 43.59% and 86.49%, respectively. The aging mouse model was successfully constructed. Compared with the aging model group, the SOD and GSH contents in the heart, liver and kidney of the wild wormwood volatile oil protection group were significantly increased (p<0.05, p<0.01, p< 0.001), MDA and PCO levels were significantly reduced (p<0.05, p<0.01, p<0.001), of which SOD content had a greater effect on the kidney, increased by 80.42%; GSH and MDA content had a greater impact on the heart, increased by 73.26% and decreased by 46.93%, respectively. The PCO content had a greater impact on the liver, which was reduced by 47.16%. It indicated that the volatile oil of Artemisia argyi has certain anti-oxidation effect in mice. The results of pathological section showed that the volatile oil of Artemisia argyi could protect the morphological integrity of oxidatively injured mouse cardiomyocytes, hepatocytes and kidney cells.