茶树菇发酵刺梨果渣制备可溶性膳食纤维工艺优化及其对小鼠润肠通便功能的评价

通过食用菌茶树菇发酵刺梨果渣,提高可溶性膳食纤维(SDF)含量,探究制备所得刺梨果渣SDF的润肠通便功能,为综合开发利用刺梨果渣膳食纤维提供依据。方法:在单因素的基础上,根据Box-Behnken实验设计对发酵培养基配方进行优化以提高SDF得率;将KM小鼠随机分为空白对照组、模型对照组、阳性对照组、SDF低(ig.0.25 g/kg·bw)、中(ig.0.50 g/kg·bw)、高剂量组(ig.1 g/kg·bw),以复方地芬诺酯建立便秘模型,连续灌胃14 d后通过测定小肠墨汁推进率、首粒黑便排出时间、6 h内排黑便粒数及重量,分析其对小鼠润肠通便功能的影响。结果:最佳制备工艺条件为氯化钙添加量1%、氯化钾添加量0.60%、糖添加量4%、液料比7:1;在此条件下刺梨果渣SDF得率为5.86%,较比未发酵原果渣SDF提高了68.78%。动物实验结果显示,在便秘模型构建成功的基础上,SDF各剂量组均有改善小鼠便秘情况的作用,其中高剂量组作用效果最明显。结论:采用食用菌茶树菇发酵刺梨果渣SDF的制备工艺合理、经济简便,所得产品具有良好的润肠通便功能。

Process Optimization of Soluble Dietary Fiber from Roxburgh rose Pomace Fermented by Agrocybe aegerita and Its Evaluation on the Moistening and Laxative Function of Mice

Rosa roxburghii pomace was fermented by Agrocybe aegerita to increase the content of soluble dietary fiber(SDF).The function of moistening intestines and defecation of Rosa roxburghii pomace was also investigated,which could provide the basis for the comprehensive development and utilization of dietary fiber of Rosa roxburghii pomace.Methods:on the basis of single factor and Box-Behnken experimental design,the fermentation medium was optimized to improve the yield of SDF.KM mice were randomly divided into blank control group,model control group,positive control group,SDF low(ig.0.25 g/kg·bw),medium(ig.0.5 g/kg·bw)and high-dose group(ig.1 g/kg·bw).The constipation model was established with compound diphenoll.After continuous intragastric administration for 14 days,the effect of the intragastric infusion on the moistening and laxative function of mice was analyzed by measuring the propulsion rate of small intestine ink,the time of the first black stool,and the number and weight of the first black stool within 6 h.Results:the optimum preparation conditions were as follows:calcium chloride of 1%,potassium chloride of 0.6%,sugar of 4%and ratio of liquid to material of 7:1.Under these conditions,the SDF yield of Rosa roxburghii pomace was 5.86%,which was 68.78%higher than that of unfermented pomace.The results of animal experiments showed that on the basis of successful constipation model,all SDF dose groups could improve the constipation of mice,and the effect of high dose group was the most obvious.Conclusion:the preparation technology of SDF from Rosa roxburghii pomace fermented by Agrocybe aegerita was reasonable,economic and convenient,and the product could have a good effect of moistening and laxative.