植物乳杆菌HG20对大鼠类风湿关节炎的改善作用

为了获得一株对类风湿性关节炎具有辅助治疗作用的乳酸菌新菌株,本研究采用形态学观察、API50 CHL糖发酵实验、16S rDNA和pheS基因序列比对,对一株分离自黄瓜表面的乳杆菌新菌株HG20进行鉴定。通过踝关节肿胀程度、血清炎症因子水平和类风湿相关指标,评价菌株HG20对Ⅱ-型胶原诱导的类风湿性关节炎模型大鼠的治疗作用。结果表明:HG20菌株被鉴定为植物乳杆菌(Lactobacillus plantarum)。动物实验结果显示,给药21 d后,HG20组大鼠踝关节肿胀周长由3.98 cm降低至1.31 cm(65.52%);足趾容积由6.61 mL降低至2.35 mL(61.28%)。与模型组比较,植物乳杆菌HG20可显著降低血清炎症因子IL-1β(38.26%)、TNF-α(52.58%)水平,提高抗炎因子IL-10表达(55.38%),减少C反应蛋白(CRP 12.89%)、免疫球蛋白IgG(46.07%)和IgM(34.29%)水平。综上所述,植物乳杆菌HG20通过降低炎症反应和调节自身免疫,对类风湿性关节炎具有辅助治疗作用,是一株有潜在应用前景的益生菌新菌株。

Improvement of Rheumatoid Arthritis with Lactobacillus plantarum HG20 in Rats

In order to obtain a new strain of lactic acid bacteria with adjuvant therapy of rheumatoid arthritis, morphological observation, API50 CHL sugar fermentation experiment, 16 S rDNA and phe S gene sequence comparison were performed to isolate a strain HG20 from the surface of cucumber for further identification. The therapeutic effect of the HG20 strain on rheumatoid arthritis model rats induced by type Ⅱ collagen was evaluated according to the indices including the degree of ankle swelling, levels of serum inflammatory factor, and rheumatoid-related indicators. The results showed that HG20 was identified as a Lactobacillus plantarum strain. Animal experiment results showed that after 21 days of administration, the ankle circumference of rats in the HG20 group decreased from 3.98 cm to 1.31 cm(by 65.52%), and the toe volume decreased from 6.61 mL to 2.35 mL(by 61.28%).Compared with the model group, L. plantarum HG20 could significantly reduce the levels of serum inflammatory cytokines(IL-1β by 38.26%;TNF-α by 52.58%), increase the expression of anti-inflammatory factor(IL-10 by 55.38%),reduce C-response protein(CRP by 12.89%) and immunoglobulin(Ig G by 46.07%;Ig M by 34.29%). In summary, L. plantarum HG20 has adjuvant therapeutic effects on rheumatoid arthritis through reducing inflammatory response and regulating autoimmunity. It is a new probiotic strain with potential application prospects.