酿酒酵母发酵生产谷胱甘肽的研究

以诱变获得的酿酒酵母突变株YF(ZnCl2r,Ethr)为试验菌株,通过摇瓶发酵、发酵罐补料分批发酵,对突变株发酵生产谷胱甘肽进行研究.确定发酵罐分批发酵的最佳培养条件为:温度30℃,pH 6.0,接种量为20%,搅拌转速为150 r/min,通气量为250 L/h.在补料分批操作方式下,分别考察了摇瓶培养、发酵罐培养...

Fermentation of Glutathione by Saccharomyces cerevisiae

Fermentation of glutathione was studied through the ways of batch fermentation and fed-batch fermentation by YF(ZnCl2r,Ethr),a test strain,which is mutant of Saccharomyces cerevisiae.The results show that the optimal fermentation condition:culture temperature 30 ℃,pH 6.0,inoculation 20%,rotate speed 150 r/min,ventilation 250 L/h.The effects of shake-flask culture and fermentor culture on GSH synthesis were studied in fed-batch fermentation.Conditions for the shake-flask culture methods are:initial sugar 10 g/L,fermentation at 4 h,10 h,16 h,22 h,28 h,respectively,glucose 25 g/L,15 g/L,5 g/L,5 gL,5 g/L added in fermentation solution,the final total glucose reached 65 g/L,the maximum cell dry weight reached 12.75 g/L,and the maximum GSH concentration and intracellular GSH content reached 76.49 mg/L and 0.59% respectively.The conditions for fermentor culture methods are:initial sugar 20 g/L,12 h fermentation of sugar begans to fill up to 24 h,the final total glucose reached 160 g/L,the maximum cell dry weight reached 30.95 g/L,the maximum GSH concentration and intracellular GSH content were 68.49 mg/L and 0.3% respectively.