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超声微波辅助法提纯菜籽蛋白的研究
引用本文:金晶,徐志宏,魏振承,刘军.超声微波辅助法提纯菜籽蛋白的研究[J].现代食品科技,2009,25(3).
作者姓名:金晶  徐志宏  魏振承  刘军
作者单位:1. 华中农业大学食品科技学院,湖北,武汉,430070;广东省农业科学院生物技术研究所,广东省农产品加工公共实验室,广东,广州,510610
2. 广东省农业科学院生物技术研究所,广东省农产品加工公共实验室,广东,广州,510610
基金项目:国家农业科技成果转化资金资助项目,广东省科技计划项目 
摘    要:选用双低菜籽粕为原料,采用超声微波辅助提取的方法,通过对各种单因素的分析得到基本工艺条件,根据Box-Benhnken的中心细合试验设计原则,选取对提取菜籽蛋白影响较大的3个因素:微波功率、提取时间和料液比值进行实验和响应面分析,得到制备菜籽蛋白的最佳工艺条件为:pH 11. 5,微波功率73 W,料液比1:28,提取时间484 s,在此条件下提取3次,产品蛋白含量83. 65%,蛋白得率38. 76%.

关 键 词:双低脱脂菜籽粕  菜籽蛋白  超声微波提取

Ultrasonic and microwave Assisted Extraction of rapeseed protein
JIN Jing,XU Zhi-hong,WEI Zhen-cheng,LIU Jun.Ultrasonic and microwave Assisted Extraction of rapeseed protein[J].Modern Food Science & Technology,2009,25(3).
Authors:JIN Jing  XU Zhi-hong  WEI Zhen-cheng  LIU Jun
Affiliation:1.The College of Food Science and Technology;Huazhong Agricultural University;Wuhan 430070;China;2.Bio-tech Institute;Guangdong Academy of Agricultural Sciences /Guangdong Open Access Laboratory of Farm Product Processing;Guangzhou 510610;China
Abstract:Application of ultrasonic and microwave assisted extraction of rapeseed protein was studied using double-low defatted rapeseed meal as a raw material by the single effect factor.The best extraction conditions were determined by Box-Benh-nken center united experimental design as follows:pH value of 11.5,microwave power of 73 W,the ratio of meal to solvent of 1:28,extracting time of 484 seconds,extracted times of 3,under which the content of protein in the extracts and its extraction rate reached 83.65% and 3...
Keywords:double-low defatted rapeseed meal  rapeseed protein  assisted by ultrasonic and microwave  
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