| 酶联免疫吸附法测定水产品中甲基睾酮残留 |
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| 引用本文： | 王强,王旭峰,黄珂,柯常亮,杨金兰,黎智广,李刘冬.酶联免疫吸附法测定水产品中甲基睾酮残留[J].现代食品科技,2015,31(5):303-308. |
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| 作者姓名： | 王强 王旭峰 黄珂 柯常亮 杨金兰 黎智广 李刘冬 |
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| 作者单位： | （中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室，农业部水产品贮藏保鲜质量安全风险评估实验室,广东广州 510300),（中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室，农业部水产品贮藏保鲜质量安全风险评估实验室,广东广州 510300),（中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室，农业部水产品贮藏保鲜质量安全风险评估实验室,广东广州 510300),（中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室，农业部水产品贮藏保鲜质量安全风险评估实验室,广东广州 510300),（中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室，农业部水产品贮藏保鲜质量安全风险评估实验室,广东广州 510300),（中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室，农业部水产品贮藏保鲜质量安全风险评估实验室,广东广州 510300),（中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室，农业部水产品贮藏保鲜质量安全风险评估实验室,广东广州 510300) |
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| 基金项目： | 国家农产品质量安全风险评估重大专项(GJFP2014009);中央级公益性科研院所基本科研业务费专项（2013TS08，2014TS09,2015TS17）；广东省食品质量安全重点实验室开放课题基金（KLFQS201202） |
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| 摘 要： | 建立了测定水产品中甲基睾酮(MT)残留的间接竞争酶联免疫吸附法(ic ELISA)。采用琥珀酸酐法衍生化制备半抗原MT17,进一步偶联钥孔血蓝蛋白(KLH)制备免疫原MT17-KLH,经动物免疫成功获取特异性识别MT的多克隆抗体(与其它结构类似物交叉反应小于1%)。优化确立了ic ELISA最佳检测条件:包被原浓度100μg/L,MT多克隆抗体稀释度1/1.2×105,抗体反应时间40 min,药物稀释液为PBS,水产样品经乙腈提取,N-丙基乙二胺吸附剂(PSA)分散固相萃取(DSPE)净化后用于ic ELISA测定。本方法的抑制中浓度IC50为3.48μg/L,检测线性范围为0.77~13.06μg/L。水产样品加标回收率为81.02~103.19%,相对标准偏差为4.46%~13.14%,测定结果与液相色谱-质谱联用法具有良好的相关性(R=0.9971)。本方法可用于水产品中甲基睾酮残留的快速测定。
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| 关 键 词： | 甲基睾酮 酶联免疫吸附法 分散固相萃取 水产品 |
| 收稿时间： | 2014/8/22 0:00:00 |
Determination of Methyltestosterone Residue in Aquatic Products by Enzyme-linked Immunosorbent Assay |
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| WANG Qiang,WANG Xu-feng,HUANG Ke,KE Chang-liang,YANG Jin-lan,LI Zhi-guang and LI Liu-dong.Determination of Methyltestosterone Residue in Aquatic Products by Enzyme-linked Immunosorbent Assay[J].Modern Food Science & Technology,2015,31(5):303-308. |
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| Authors: | WANG Qiang WANG Xu-feng HUANG Ke KE Chang-liang YANG Jin-lan LI Zhi-guang and LI Liu-dong |
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| Affiliation: | (South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, Laboratory of Quality and Safety Risky Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture, Guangzhou 510300, China),(South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, Laboratory of Quality and Safety Risky Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture, Guangzhou 510300, China),(South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, Laboratory of Quality and Safety Risky Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture, Guangzhou 510300, China),(South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, Laboratory of Quality and Safety Risky Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture, Guangzhou 510300, China),(South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, Laboratory of Quality and Safety Risky Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture, Guangzhou 510300, China),(South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, Laboratory of Quality and Safety Risky Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture, Guangzhou 510300, China) and (South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Aquatic Product Processing, Ministry of Agriculture, Laboratory of Quality and Safety Risky Assessment for Aquatic Product on Storage and Preservation, Ministry of Agriculture, Guangzhou 510300, China) |
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| Abstract: | |
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| Keywords: | methyltestosterone enzyme-linked immunosorbent assay dispersive solid phase extraction aquatic product |
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