| 鲶鱼体表粘液粗提物对铜绿假单胞菌的抑菌机理初探 |
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| 引用本文： | 李婷婷,张慧芳,姜杨,晋高伟,励建荣.鲶鱼体表粘液粗提物对铜绿假单胞菌的抑菌机理初探[J].现代食品科技,2015,31(7):67-73. |
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| 作者姓名： | 李婷婷 张慧芳 姜杨 晋高伟 励建荣 |
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| 作者单位： | （1.大连民族学院生命科学学院，辽宁大连 116600）（2.西南大学食品学院，重庆 400715）,（3.渤海大学食品科学研究院，辽宁省食品安全重点实验室，辽宁锦州 121013）,（3.渤海大学食品科学研究院，辽宁省食品安全重点实验室，辽宁锦州 121013）,（3.渤海大学食品科学研究院，辽宁省食品安全重点实验室，辽宁锦州 121013）,（2.西南大学食品学院，重庆 400715）（3.渤海大学食品科学研究院，辽宁省食品安全重点实验室，辽宁锦州 121013） |
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| 基金项目： | 国家自然科学基金（31301572，31471639）；中国博士后科学基金（2014M552302）；“十二五”国家科技支撑计划（2012BAD29B06）；重庆市博士后资助(Xm2014041) |
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| 摘 要： | 本文以铜绿假单胞菌为研究对象,探讨鲶鱼体表粘液提取物(Catfish Epidermal Mucus Extracts,CEME)对其的抑制效果及抑菌机理。研究表明:当CEME浓度高于0.15%时,鲶鱼体表粘液提取物对铜绿假单胞菌有显著的抑制作用,通过测定微生物的生长曲线可以看出CEME能够有效减缓铜绿假单胞菌的生长速率。进一步使用扫描电镜发现CEME对铜绿假单胞菌的细胞形态结构能够造成明显的损伤,有效破坏微生物细胞膜的完整性。同时CEME还可以导致铜绿假单胞菌细胞膜的通透性增加,从而导致体内小分子物质以及部分大分子物质向外泄漏。应用SDS-PAGE方法发现CEME处理对铜绿假单胞菌的总蛋白和细菌膜蛋白均有显著的影响,它能够抑制菌体某些蛋白的合成,导致胞内蛋白含量的下降和缺失。上述研究结果表明鲶鱼体表粘液提取物对铜绿假单胞菌有较好的抑制效果。
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| 关 键 词： | 鲶鱼体表粘液提取物 铜绿假单胞菌 抑菌机理 研究 |
| 收稿时间： | 2014/10/15 0:00:00 |
Antibacterial Mechanisms of Catfish Epidermal Mucus Extracts against Pseudomonas aeruginosa |
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| LI Ting-ting,ZHANG Hui-fang,JIANG Yang,JIN Gao-wei and LI Jian-rong.Antibacterial Mechanisms of Catfish Epidermal Mucus Extracts against Pseudomonas aeruginosa[J].Modern Food Science & Technology,2015,31(7):67-73. |
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| Authors: | LI Ting-ting ZHANG Hui-fang JIANG Yang JIN Gao-wei and LI Jian-rong |
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| Affiliation: | (1.College of Life Science, Dalian Nationality of University, Dalian 116600, China)
(2.College of Food Science, Southwest University, Chongqing 400715, China),(3.Food Science Research Institute of Bohai University, Food Safety Key Lab of Liaoning Province, Jinzhou 121013, China),(3.Food Science Research Institute of Bohai University, Food Safety Key Lab of Liaoning Province, Jinzhou 121013, China),(3.Food Science Research Institute of Bohai University, Food Safety Key Lab of Liaoning Province, Jinzhou 121013, China) and (2.College of Food Science, Southwest University, Chongqing 400715, China)
(3.Food Science Research Institute of Bohai University, Food Safety Key Lab of Liaoning Province, Jinzhou 121013, China) |
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| Abstract: | Several techniques were used to investigate the mechanism of antibacterial effect of catfish epidermal mucus extracts (CEME) against Pseudomonas aeruginosa. The results showed that the inhibitory effect was significant when the concentration exceeded 0.15% (mg/mL). The bacterial growth curve showed a decrease and scanning electron microscopy (SEM) showed changes in morphology. This indicated that the primary mechanism of CEME inhibitory action was to damage the bacterial cell membrane integrity. CEME also increased bacterial membrane permeability, which led to leakage of cellular small molecules and a proportion of macromolecules. In addition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) revealed that CEME causes obvious changes to bacterial membrane proteins, which led to inhibition of bacterial protein synthesis and subsequent decline and loss of intracellular protein content. These results imply that CEME shows relatively good antibacterial effect on Pseudomonas aeruginosa. |
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| Keywords: | catfish epidermal mucus extracts Pseudomonas aeruginosa antibacterial mechanisms research |
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