| 应用LAMP实时浊度法检测转基因大豆 |
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| 引用本文： | 袁瑛娜,单潇潇,王宗德,石磊,袁秀金,谭贵良.应用LAMP实时浊度法检测转基因大豆[J].现代食品科技,2011(10):1264-1267. |
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| 作者姓名： | 袁瑛娜 单潇潇 王宗德 石磊 袁秀金 谭贵良 |
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| 作者单位： | 江西农业大学园林与艺术学院;华南理工大学生物科学与工程学院;华南理工大学轻工与食品学院;广东省中山市质量计量监督检测所; |
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| 基金项目： | 广东省质监局科技计划项目(2009zz11) |
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| 摘 要： | DNA环介导等温扩增(Loop Mediated Isothermal Amplification,LAMP)方法是一种新型的核酸扩增检测方法,该方法操作简便、所需时间短、灵敏度高、特异性强.实时浊度法可以实时检测反应过程中所产生的白色沉淀,从而实现对LAMP整个反应过程的实时监控.本研究以抗草甘膦转基因大豆为研究对象...
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| 关 键 词： | 环介导等温核酸扩增技术 实时浊度仪 转基因大豆 检测 |
Development of a Real-time Turbidimeter-based Loop-mediated Isothermal Amplification Assay for Detection of Transgenic Soybean |
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| YUAN Ying-na,SHAN Xiao-xiao,WANG Zong-de,SHI Lei,YUAN Xiu-jin,TAN Gui-liang.Development of a Real-time Turbidimeter-based Loop-mediated Isothermal Amplification Assay for Detection of Transgenic Soybean[J].Modern Food Science & Technology,2011(10):1264-1267. |
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| Authors: | YUAN Ying-na SHAN Xiao-xiao WANG Zong-de SHI Lei YUAN Xiu-jin TAN Gui-liang |
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| Affiliation: | YUAN Ying-na1,SHAN Xiao-xiao2,WANG Zong-de1,SHI Lei3,YUAN Xiu-jin4,TAN Gui-liang4(1.College of Landscape and Art,Jiangxi Agricultural University,Nanchang 330045,China)(2.School of Biological Science and Engineering,South China University of technology,Guangzhou 510006,China)(3.School of Light Industry and Food Science,Guangzhou510006,China)(4.Zhongshan Supervision Testing Institute of Quality & Metrology,Zhongshan 528403,China) |
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| Abstract: | Loop-mediated isothermal amplification method(LAMP) is a novel nucleic acid ampli-fication technology.The LAMP method amplifies DNA with rapidity,high specificity and sensitivity under isothermal conditions.Since turbidity of the reaction mixture would increase in correlation with the DNA yield,real-time monitoring of the LAMP reaction was achieved by real-time turbidimeter.Enolpyruvl Shimimate phosphate syntheses gene was amplified by a set of four specially primers that recognize six distinct sequences of... |
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| Keywords: | LAMP real-time turbidimeter transgenic soybean detection |
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