不同分子量海鲈鱼胶原蛋白肽组分的功能特性比较

本文采用不同截留分子量的超滤膜对海鲈鱼胶原蛋白肽溶液进行分级分离,采用碱性蛋白酶水解海鲈鱼加工副产物中提取的胶原蛋白制得的胶原蛋白肽溶液分成三个不同分子量组分SBCP1(Mw2000 u)、SBCP2(2000 uMw3000 u)和SBCP3(Mw3000 u),比较了海鲈鱼胶原蛋白肽它们的吸水性、持水性、溶解性、吸油性、乳化性和乳化稳定性、起泡性和起泡稳定性等功能特性。结果显示:小分子量的SBCP1组分的吸水性和溶解性最好;大分子量SBCP3组分的持水性、乳化性和乳化稳定性以及起泡性和泡沫稳定性最好;中等分子量的SBCP2组分的吸油性最好,这个结果提示了海鲈鱼胶原蛋白肽不同分子量组分的理化特性存在显著差异,其功能特性和分子量分布有密切关系。本研究为海鲈鱼胶原蛋白肽产品的开发利用提供了指导。

Comparing the Functional Properties of Sea Bass Collagen Peptides (SBCP) with Different Molecular Weights

In this study, sea bass collagen peptide (SBCP) solution was fractionated using ultrafiltration membranes with different molecular weight cutoffs (Mw COs). The enzymatic hydrolysis of collagen peptide extracted from sea bass processing byproducts yielded collagen peptide solution, which was separated into three components with different molecular weights (Mw s): SBCP1 (Mw < 2000 u), SBCP2 (2000 u < Mw <3 000 u), and SBCP3 (Mw > 3000 u). The water absorption capacity, water holding capacity, solubility, oil absorption, emulsifying properties, emulsion stability, foaming properties, and foaming stability of those components were also compared. SBCP1 showed the highest water absorption capacity and solubility; SBCP3 had the highest water holding capacity, emulsifying properties, emulsion stability, foaming properties, and foam stability; SBCP2 showed the highest capacity for oil absorption. This result suggested that there was a significant difference in physicochemical characteristics among components of SBCP with different Mw s and that the functional properties were closely related to the Mw distribution. This study provides guidance for the development and application of SBCP products.