芦荟白酒毒性评价及对酒精性肝损伤小鼠的保护作用研究

研究芦荟白酒的急性和亚慢性毒性及其对酒精性肝损伤小鼠的保护作用。采用一次性经口急性毒性试验比较了芦荟白酒和基酒的安全性差异;通过建立酒精性肝损伤模型,测定小鼠血清、肝脏生化指标和观察肝组织病理学变化,考察了芦荟白酒对酒精性肝损伤小鼠的保护作用。芦荟白酒的半数致死量(LD50)及其95%,99%平均可信限均比基酒大,表明芦荟白酒比基酒安全,急性毒性小;与基酒相比,芦荟白酒能显著降低血清中的谷草转氨酶(AST)、谷丙转氨酶(ALT)、胆固醇(TC)、甘油三酯(TG)水平,提高肝组织超氧化物岐化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-PX)水平,降低肝脏AST、ALT及丙二醛(MDA)含量。低、中剂量效果最佳。肝组织病理学检测结果证明,适量的芦荟白酒对酒精性肝损伤小鼠有保护作用,其作用机理与抑制机体脂质过氧化、增强肝脏抗氧化能力有关。

Evaluating Toxicity and Protective Effects of Aloe Liquor in Mice with Alcohol-induced Liver Injury

The acute and sub-chronic toxicity and hepatoprotective activity of aloe liquor was studied in mice with alcohol-induced liver injury. The difference between health risks of aloe liquor and base liquor were evaluated by a one-time acute oral toxicity test. A mouse model of alcohol-induced liver injury was established to measure serum and liver biochemical indices, observe histopathological changes in the liver, and determine the hepatoprotective effect of aloe liquor on mice with alcohol-induced liver injury. Both the median lethal dose (LD50) and 95% (or 99%) confidence interval of aloe liquor were higher than the values of base liquor, indicating that the former is safer and has a low level of toxicity. Compared with base liquor, aloe liquor could significantly reduce the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total cholesterol (TC), and total triglyceride (TG), while increase superoxide dismutase (SOD), glutathione (GSH), and glutathione peroxidase (GSH-PX) activities, and reduce the contents of AST, ALT, and malondialdehyde (MDA) in liver tissue. The optimal efficacy for alleviating liver injury were seen in mice treated with low- and medium-dose aloe liquor. The liver histological observations demonstrated that optimal amounts of aloe liquor exhibited hepatoprotective effects in mice with alcohol-induced liver injury, and the protective mechanism is related to the inhibition of lipid peroxidation and enhancement of liver antioxidant capacity.