| 转谷氨酰胺酶酶原在枯草芽孢杆菌WB800中的表达 |
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| 引用本文： | 罗宁,杨慧林,沈徐凯,郑明英.转谷氨酰胺酶酶原在枯草芽孢杆菌WB800中的表达[J].现代食品科技,2011,27(7):734-737. |
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| 作者姓名： | 罗宁 杨慧林 沈徐凯 郑明英 |
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| 作者单位： | 1. 广东肇庆星湖生物科技股份有限公司,广东肇庆,526060
2. 华南理工大学生物科学与工程学院,广东广州,510006 |
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| 基金项目： | 广东省科技攻关项目(2006B13001006) |
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| 摘 要： | 以茂原链霉菌(Streptomyces mobaraensis)基因组 DNA 为模板,PCR扩增得到转谷氨酰胺酶酶原基因(pro-transglutaminase,pro-TG),经pMD18-T载体亚克隆到表达载pBEp43(+),然后转化到枯草芽孢杆菌(Bacillus subtilis) WB800中,经过32...
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| 关 键 词： | 转谷氨酰胺酶酶原 枯草芽孢杆菌 分泌表达 亲和层析 BSA交联 |
Expression of pro-transglutaminase in Bacillus subtilis WB800 |
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| LUO Ning,YANG Hui-lin,SHEN Xu-kai,ZHANG Ming-ying.Expression of pro-transglutaminase in Bacillus subtilis WB800[J].Modern Food Science & Technology,2011,27(7):734-737. |
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| Authors: | LUO Ning YANG Hui-lin SHEN Xu-kai ZHANG Ming-ying |
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| Affiliation: | LUO Ning1,YANG Hui-lin2,SHEN Xu-kai2,ZHENG Ming-ying1(1.Star Lake Bioscience Co.,Ltd,Zhaoqing 526060,China)(2.School of Bioscience and Bioengineering,South China University of Technology,Guangzhou 510006,China) |
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| Abstract: | The pro-transglutaminase(pro-TG) gene was obtained by PCR amplification using Streptomyces mobaraensis genome DNA as template and subcloned into the expression plasmid pBEP43(+) via pMD18-T vector,then the plasmid was transformed into B.subtilis WB800.After 32 h flask fermentation,pro-TG protein was successfully expressed in the culture broth.Mature-transglutaminase was obtained after cutting the leader peptide by trypsin activation.BSA cross-linking experiment shows that the mature transglutaminase has cro... |
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| Keywords: | pro-transglutaminase Bacillus subtilis secretory expression affinity chromatography BSA cross-linking |
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